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リコンビナントタンパク質・酵素

Recombinant BRPF3 (576-701) protein

Aliases: Bromodomain And PHD Finger Containing 3, KIAA1286
商品コード: 31487 Format: 100 µg ¥73,000 Buy Now
商品コード: 31787 Format: 1 mg ¥481,000 Buy Now
Expressed In: E. coli Protein Species: Human

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for Recombinant BRPF3 (576-701) proteinContents

Recombinant BRPF3 (576-701) protein expressed in E. coli cells and is provided in 25 mM Tris pH 7.4, 150 mM NaCl, 5% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant BRPF3 (576-701) proteinBackground

BRPF3 (Bromodomain and PHD finger containing 3) is a component of the MOZ/MORF histone acetyltransferase (HAT) complex. The acetylation of histone lysine residues plays a crucial role in the epigenetic regulation of gene transcription. Acetylated lysine residues can be recognized by bromodomain. The bromodomains serve as “readers” of histone acetylation marks regulating the transcription of target promoters. There is less study on its function now.

for Recombinant BRPF3 (576-701) proteinApplication Notes

Recombinant BRPF3 (576-701) is suitable for use in binding assays, inhibitor screening, and selectivity profiling.

for Recombinant BRPF3 (576-701) proteinProtein Details

The peptide corresponding to amino acids 576-701 that contains the bromodomain sequences of BRPF3 (accession number NM_015695.2) was expressed in E. coli and contains an N-terminal His-Tag and C-terminal FLAG-Tag with an observed molecular weight of 19.7 kDa. The recombinant protein is >90% pure by SDS-PAGE.

 
Recombinant BRPF3 (576-701) protein

Recombinant BRPF3 (576-701) protein gel.
BRPF3 (576-701) protein was run on an SDS-PAGE gel and stained with Coomassie blue.

BRPF3 (576-701) HTRF activity assay

Recombinant BRPF1 (576-701) HTRF activity assay.
3.3 uM histone peptide H4K5/8/12/16(4Ac) was incubated with BRPF1 (576-701) protein in reaction buffer including 50mM HEPES-NaOH pH 7.0, 0.1% BSA overnight at room temperature. Anti-Flag antibody was used to detect reaction products.

for Recombinant BRPF3 (576-701) proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant BRPF3 (576-701) proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant BRPF3 (576-701) protein
 

Data Thumbnails

Recombinant BRPF3 (576-701) protein

Recombinant BRPF3 (576-701) Coomassie stained gel.
(Click image to enlarge and see details).

BRPF3 (576-701) HTRF activity assay

BRPF3 (576-701) HTRF activity assay.
(Click image to enlarge and see details).