Active Motif,
Tools to analyze nuclear function,
Your CartYour Cart 0 items
リコンビナントタンパク質・酵素

Recombinant BRD9 (130-259), GST-Tag

Aliases: PRO9856, LAVS3040
商品コード: 31488 Format: 100 µg ¥73,000 Buy Now
商品コード: 31788 Format: 1 mg ¥537,000 Buy Now
Expressed In: E. coli Protein Species: Human

Request a quote for a bulk orderBulk Order Button


for Recombinant BRD9 (130-259), GST-TagContents

Recombinant BRD9 (130-259), GST-Tag was expressed in E. coli cells and is supplied in 25 mM Tris pH 7.4, 150 mM NaCl, 5% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant BRD9 (130-259), GST-TagBackground

BRD9 (Bromodomain-containing protein 9) belongs to the BET subclass of proteins, which are characterized by two N-terminal bromodomains and one ET (Extra Terminal) domain. BRDs associate with chromatin through their bromodomains that recognize acetylated histone lysine residues. Bromodomains function as ‘readers’ of these epigenetic histone marks and regulate chromatin structure and gene expression by linking associated proteins to the acetylated nucleosomal targets. The ET domain functions as a protein binding motif and exerts atypical serine-kinase activity. The BET family consists of at least four members in mouse and human, BRD2 (also referred to as FSRG1, RING3), BRD3 (FSRG2, ORFX), BRD4 (FSRG4, MCAP/HUNK1), and BRDT (FSRG3, BRD6) that function in the regulation of transcriptional activation and chromatin remodeling. There are five isoforms of BRD9 that are produced by alternative splicing.

for Recombinant BRD9 (130-259), GST-TagApplication Notes

Recombinant BRD9 (130-259) is suitable for use in binding assays, inhibitor screening, and selectivity profiling.

for Recombinant BRD9 (130-259), GST-TagProtein Details

The peptide corresponding to amino acids 130 - 259 that contains the bromodomain sequences of BRD9 (accession number NM_023924.4) was expressed in E. coli and contains an N-terminal GST-Tag with an observed molecular weight of 41 kDa. The recombinant protein is >95% pure by SDS-PAGE.

 
BRD9 (130-259) protein Coomassie gel

Recombinant BRD9 (130-259) SDS-PAGE gel.
BRD9 (130-259) protein was run on a 12% SDS-PAGE gel and stained with Coomassie blue.

BRD9 (130-259) HTRF activity assay

Recombinant BRD9 (130-259) HTRF activity assay.
3.3 uM histone peptide H4K5/8/12/16(4Ac) was incubated with BRD9 (130-259), GST-Tag in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-GST antibody was used to detect reaction products

for Recombinant BRD9 (130-259), GST-TagStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant BRD9 (130-259), GST-TagGuarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

Technical Data Sheet

Recombinant BRD9 (130-259), GST-Tag
 

Data Thumbnails

BRD9 (130-259) protein Coomassie gel

BRD9 (130-259) protein Coomassie gel.
(Click image to enlarge and see details).

BRD9 (130-259) HTRF activity assay

BRD9 (130-259) HTRF activity assay.
(Click image to enlarge and see details).