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Recombinant HDAC2 protein

Aliases: Histone Deacteylase 2, HD2, RPD3, YAF1
商品コード: 31505 Format: 50 µg ¥73,000 Buy Now
商品コード: 31909 Format: 1 mg ¥648,000 Buy Now
Expressed In: Baculovirus Protein Species: Human

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for Recombinant HDAC2 proteinContents

Full length recombinant HDAC2 protein was expressed in Sf9 cells and is supplied in 25 mM Hepes pH 7.5, 300 mM NaCl, 5% Glycerol, 0.04% Triton X-100, 0.2 mM TCEP, 0.2 mg/ml 3× Flag peptide. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant HDAC2 proteinBackground

HDAC2 (Histone Deacetylase 2, also designated mammalian RPD3) is a member of the class I mammalian histone deacetylases (HDACs) involved in regulating chromatin structure during transcription. These enzymes catalyze the removal of acetyl groups from lysine residues of histones and other cellular proteins. Lysine N-ε-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in regulation of gene expression in various cellular functions. It consists of the transfer of an acetyl moiety from an acetyl coenzyme A to the ε-amino group of a lysine residue.

In vivo, acetylation is controlled by the antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs). The HDACs are grouped into four classes, on the basis of similarity to yeast counterparts: HDAC class I (HDAC1, HDAC2, HDAC3 and HDAC8), class II (HDAC4, HDAC5, HDAC6, HDAC7, 9 and 10), class III (SIRT1-7) and class IV (HDAC11). HDAC2 is associated with many different proteins as YY1 (a mammalian zinc-finger transcription factor). HDAC2 also forms transcriptional repressor complexes containing, among others, HDAC1 or RBBP4. HDAC1, HDAC2 and HDAC3 are also ubiquitously expressed and can deacetylate both H3 and H4 in free histones or nucleosome substrate.

for Recombinant HDAC2 proteinApplication Notes

Recombinant HDAC2 is suitable for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Active Motif offers a variety of protein screening tools including HDAC assay kits.

HDAC Activity Assay Conditions: 3 μM Histone H3K9ac (1-21aa) peptide was incubated with HDAC2 protein in reaction buffer including 25 mM Tris-HCl pH 8.0, 137 mM NaCl, 2.7 mM MgCl2, 1 mM KCl and 0.1 mg/ml BSA for 30 min at 37°C. HTRF assay was used for activity detection.

for Recombinant HDAC2 proteinProtein Details

Recombinant human HDAC2 was expressed in Sf9 cells as the full length protein (accession number NP_001518.3) with a C-terminal FLAG-Tag. The molecular weight of the protein is 60 kDa. The recombinant protein is >80% pure by SDS-PAGE.

 
HDAC2 protein Coomassie gel

Recombinant HDAC2 protein gel.
HDAC2 protein was run on an 8% SDS-PAGE gel and stained with Coomassie blue.

HDAC2 protein activity assay

Recombinant HDAC2 activity assay.
3 μM Histone H3K9ac (1-21aa) peptide was incubated with different concentrations of HDAC2 protein in reaction buffer for 30 min at 37°C, Reaction product was detected by Anti-H3K9me0-Eu antibody. HTRF assay was used for activity detection.

for Recombinant HDAC2 proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant HDAC2 proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant HDAC2 protein
 

Data Thumbnails

HDAC2 protein Coomassie gel

HDAC2 protein Coomassie gel.
(Click image to enlarge and see details).

HDAC2 protein activity assay

HDAC2 protein activity assay.
(Click image to enlarge and see details).