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リコンビナントタンパク質・酵素

Recombinant HDAC6 (597-728, deleted mutant) protein

Aliases: HD6, JM21, CPBHM, PPP1R90
商品コード: 31506 Format: 20 µg ¥73,000 Buy Now
Expressed In: Baculovirus Protein Species: Human

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for Recombinant HDAC6 (597-728, deleted mutant) proteinContents

Recombinant HDAC6 (597-728, deleted mutant) is supplied in 25 mM Hepes pH 7.5, 300 mM NaCl, 5% Glycerol, 0.04% Triton X-100, 0.2 mM TCEP, 0.2 mg/ml 3× FLAG peptide. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant HDAC6 (597-728, deleted mutant) proteinBackground

HDAC6 (Histone Deacetylase 6) is a member of the class IIb mammalian histone deacetylases (HDACs) involved in regulating chromatin structure during transcription. These enzymes catalyze the removal of acetyl groups from lysine residues of histones or many cellular proteins. Lysine N-ε-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in regulation of gene expression in various cellular functions. It consists of the transfer of an acetyl moiety from an acetyl coenzyme A to the ε-amino group of a lysine residue.

In vivo, acetylation is controlled by the antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs). The HDACs are grouped into four classes, on the basis of similarity to yeast counterparts: HDAC class I (HDAC1, HDAC2, HDAC3 and HDAC8), class II (HDAC4, HDAC5, HDAC6, HDAC7, 9 and 10), class III (SIRT1-7) and class IV (HDAC11).

HDAC6 is a unique enzyme that harbors a full duplication of its deacetylase homology region, which appears to contribute independently to the overall activity of HDAC6 protein. Alpha-tubulin and HSP90 are two known substrates of HDAC6, playing an important role in cellular mechanisms related to the microtubule network and HSP90-dependent events. HDAC6 also participates in regulating expression of a group of genes involved in the remodeling of chromatin during cell differentiation.

for Recombinant HDAC6 (597-728, deleted mutant) proteinApplication Notes

Recombinant HDAC6 (597-728, deleted mutant) protein is suitable for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

HDAC Activity Assay Conditions: 3 μM H3K9ac (1-21) peptides was incubated with different concentrations of HDAC6 (597-728, deleted mutant) in 25 mM Tris-HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 0.1 mg/ml BSA for 30 minutes at 37°C followed by developing for 30 minutes at room temperature.

for Recombinant HDAC6 (597-728, deleted mutant) proteinProtein Details

Recombinant HDAC6 (597-728, deleted mutant) deletes the second catalytic domain. This recombinant protein was expressed in a baculovirus expression system (accession number NP_006035.2) with an N-terminal 6×His-Tag and FLAG-Tag. The molecular weight of the protein is 122.1 kDa. The recombinant protein is >80% pure by SDS-PAGE.

 
HDAC6 protein Coomassie gel

Recombinant HDAC6 (597-728, deleted mutant) protein gel.
HDAC6 protein was run on an 8% SDS-PAGE gel and stained with Coomassie blue.

HDAC6 protein activity assay

Recombinant HDAC6 (597-728, deleted mutant) protein activity assay.
3 μM H3K9ac (1-21) peptides was incubated with different concentrations of HDAC6 (597-728, deleted mutant) in reaction buffer for 30 minutes at 37°C followed by developing for 30 minutes at room temperature.

for Recombinant HDAC6 (597-728, deleted mutant) proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant HDAC6 (597-728, deleted mutant) proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant HDAC6 (597-728, deleted mutant) protein
 

Data Thumbnails

HDAC6 protein Coomassie gel

HDAC6 protein Coomassie gel.
(Click image to enlarge and see details).

HDAC6 protein activity assay

HDAC6 protein activity assay.
(Click image to enlarge and see details).