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Recombinant ACF complex

商品コード: 31509 Format: 20 µg ¥77,000 Buy Now
商品コード: 31913 Format: 1 mg ¥703,000 Buy Now
Expressed In: Baculovirus Protein Species: Drosophila melanogaster

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for Recombinant ACF complexContents

Recombinant ACF complex is supplied in 25 mM HEPES pH 7.5, 300 mM NaCl, 5% glycerol, 0.04% Triton X-100, 0.2 mM TCEP. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant ACF complexBackground

ACF Complex (ATP-utilizing chromatin assembly and remodeling factor protein complex) which contains Acf1 and Iswi catalyzes the ATP-dependent assembly of periodic nucleosome arrays in vitro. The catalytic subunit of ACF is the ISWI ATP-dependant chromatin remodeling protein. ACF is related to CHRAC (Chromatin Accessibility Complex), which also contains Acf1 and Iswi. ACF is involved in the establishment and possibly maintenance of silenced heterochromatic domains in the genome, both at pericentric heterochromatin and at genes repressed by the Polycomb complex.

for Recombinant ACF complexApplication Notes

Recombinant ACF complex is suitable for in vitro chromatin assembly.

for Recombinant ACF complexProtein Details

Recombinant ACF complex that includes full length Drosophila Acf1 and Iswi (accession numbers NP_536734.2 and NP_523719.1, respectively) was expressed in Sf9 cells and contains an FLAG-Tag at the N-terminus of Acf1. The molecular weights of expressed Acf1 and Iswi are 172 kDa and 119 kDa, respectively.

 
ACF complex Coomassie gel

Recombinant ACF complex gel
ACF complex was run on an 8% SDS-PAGE gel and stained with Coomassie Blue.
MW: Acf1: 172 kDa. Iswi: 119 kDa.
Purity: > 75%.

for Recombinant ACF complexStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant ACF complexGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant ACF complex
 

Data Thumbnails

ACF complex Coomassie gel

ACF complex Coomassie gel.
(Click image to enlarge and see details).