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リコンビナントタンパク質・酵素

Recombinant PRDM9 (191-414) protein

Aliases: PFM6, MSBP3, ZNF899, MEISETZ
商品コード: 31510 Format: 20 µg ¥73,000 Buy Now
Expressed In: E. coli Protein Species: Human

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for Recombinant PRDM9 (191-414) proteinContents

Recombinant PRDM9 protein was expressed in E.coli and is supplied in 20 mM Tris pH 7.5, 300 mM NaCl, 0.5 mM TCEP, 5 % glycerol, 1 μM ZnCl2. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant PRDM9 (191-414) proteinBackground

PRDM9 (PR Domain Containing 9) is a Histone methyltransferase that specifically trimethylates Lys-4 of histone H3 during meiotic prophase and is essential for proper meiotic progression. PRDM9 has the ability to mono- and dimethylate Lys-4 of histone H3. H3 Lys-4 methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes during early meiotic prophase (By similarity).

for Recombinant PRDM9 (191-414) proteinApplication Notes

Recombinant PRDM9 is suitable for use in enzyme kinetics, inhibitor screening, and selectivity profiling.

HMT Assay Conditions: 50 mM TrisCl, pH 8.5, 30 mM NaCl, 5% glycerol, 2 mM DTT, 5, 25, and 50 μM H3 (1-24) peptide, 100 nM Recombinant PRMT5 protein at room temperature.

for Recombinant PRDM9 (191-414) proteinProtein Details

Recombinant PRDM9 protein contains amino acids 191 - 414 of the human PRDM9 (accession number NP_064612.2) with a GST-Tag and expressed in E.coli with a molecular weight of 25.6 kDa.

 
PRDM9 protein Coomassie gel

Recombinant PRDM9 protein gel.
PRDM9 protein was run on a 12% SDS-PAGE gel and stained with Coomassie blue.

PRDM9 protein protein activity assay

Recombinant PRDM9 protein activity assay.
PRDM9 protein was used in an HMT assay to determine enzyme acticvity. Activity was detected by MALDI-TOF.

for Recombinant PRDM9 (191-414) proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant PRDM9 (191-414) proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Technical Data Sheet

Recombinant PRDM9 (191-414) protein
 

Data Thumbnails

PRDM9 protein Coomassie gel

PRDM9 protein Coomassie gel.
(Click image to enlarge and see details).

PRDM9 protein protein activity assay

PRDM9 protein activity assay.
(Click image to enlarge and see details).