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リコンビナントタンパク質・酵素

Recombinant EHMT1 (894-1298) protein

Aliases: Euchromatic Histone Lysine Methyltransferase 1, Histone H3-K9 Methyltransferase, G9a-Like Protein, H3-K9-HMTase 5, KMT1D, GLP1, EuHMT1, EuHMTase1, G9a like protein
商品コード: 31520 Format: 50 µg ¥73,000 Buy Now
商品コード: 31920 Format: 1 mg ¥537,000 Buy Now
Expressed In: E. coli Protein Species: Human

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for Recombinant EHMT1 (894-1298) proteinContents

Recombinant EHMT1 (894-1298) protein is supplied in 25 mM Tris-HCl pH 8.0, 300 mM NaCl, 5% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant EHMT1 (894-1298) proteinBackground

EHMT1(Euchromatic Histone Methyltransferase 1, EuHMT1, EuHMTase1, G9a like protein, GLP1) is a SET domain-containing histone methyltransferase specific for lysine 9 of histone H3. It is possible that this protein is involved in the silencing of Myc and E2F-responsive genes and thus could play a role in regulating cell cycle transitions from G0 to G1.

for Recombinant EHMT1 (894-1298) proteinApplication Notes

Recombinant EHMT1 protein is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.

HMT Assay Conditions: 3 µM H3 (1-21) peptide was incubated with different concentrations of EHMT1 (894-1298) protein in 10 μl reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP for 3 hours, then 10 µl H3K9me2 antibody and SA-XL665 mixture (each 1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.

for Recombinant EHMT1 (894-1298) proteinProtein Details

Recombinant EHMT1 (894-1298) protein that includes amino acids 894-1298 of human EHMT1 protein (accession number NP_079033.4) was expressed in E. coli and contains an N-terminal GST-Tag with a molecular weight of 79.1 kDa. The purity of the protein is > 85% by SDS-PAGE.

 
EHMT1 (894-1298) protein Coomassie Blue gel.

Recombinant EHMT1 (894-1298) SDS-PAGE gel.
Recombinant EHMT1 was run on a 10% SDS-PAGE gel and stained with Coomassie Blue.
MW: 79.1 kDa
Purity: > 85%

HTRF assay for EHMT1 (894-1298) activity

HTRF assay for EHMT1 (894-1298) activity
3 µM histone peptide H3 (1-21) was incubated with EHMT1 (894-1298) in reaction buffer for 3 hours at room temperature. H3K9me2 antibody was used to detect reaction product.

HTRF assay for EHMT1 (894-1298) activity

HTRF assay for EHMT1 (894-1298) activity
3 µM histone peptide H3 (1-21) was incubated with EHMT1 (894-1298) in reaction buffer for 3 hours at room temperature. H3K9me2 antibody was used to detect reaction product.

for Recombinant EHMT1 (894-1298) proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant EHMT1 (894-1298) proteinGuarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

 

Technical Data Sheet

Recombinant EHMT1 (894-1298) protein
 

Data Thumbnails

EHMT1 (894-1298) protein Coomassie Blue gel.

EHMT1 (894-1298) protein Coomassie stained gel.
(Click image to enlarge and see details).

HTRF assay for EHMT1 (894-1298) activity

HTRF assay for EHMT1 (894-1298) activity
(Click image to enlarge and see details).

HTRF assay for EHMT1 (894-1298) activity

HTRF assay for EHMT1 (894-1298) activity
(Click image to enlarge and see details).