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リコンビナントタンパク質・酵素

Recombinant DNMT3A (278-432) protein

Aliases: DNA Methyltransferase 3A, DNMT3A2, TBRS, M.HsaIIIA
商品コード: 31541 Format: 100 µg ¥73,000 Buy Now
商品コード: 31941 Format: 1 mg ¥537,000 Buy Now
Expressed In: E. coli Protein Species: Human

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for Recombinant DNMT3A (278-432) proteinContents

Recombinant DNMT3A (278-432) was expressed in E. coli cells and is supplied in 25 mM Tris pH 7.4, 150 mM NaCl, 5% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant DNMT3A (278-432) proteinBackground

DNMT3A (DNA methyltransferase 3 alpha) is a de novo methyltransferase. DNMT3A is involved in DNA methylation, in which a methyl group is added to a cytosine residue on DNA, commonly at the C5 position of a CpG dinucleotide. DNMT3A has been shown to be important in the regulation of specific patterns of DNA methylation. Methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as embryonic development, genetic imprinting, X chromosome inactivation and control of gene expression. DNA methylation is generally associated with transcriptional repression.

Trimethylation of Lys36 on histone H3 tail (H3K36me3) is associated with DNA methylation and elongation phase of transcription. PWWP domain of DNMT3A reads this epigenetic mark to guide DNA methylation.

for Recombinant DNMT3A (278-432) proteinApplication Notes

Recombinant DNMT3A (278-432) is suitable for use in binding assays, inhibitor screening, and selectivity profiling. 

for Recombinant DNMT3A (278-432) proteinProtein Details

The peptide corresponding to amino acids 278 - 432 that contains the PWWP sequences of DNMT3A (accession number NP_072046.2) was expressed in E. coli and contains an N-terminal GST-Tag with an observed molecular weight of 43.5 kDa. The recombinant protein is >90% pure by SDS-PAGE.

 
Recombinant DNMT3A (278-432), GST-Tag gel.

Recombinant DNMT3A (278-432) protein gel.
Recombinant DNMT3A (278-432) protein was run on an 8% SDS-PAGE gel and stained with Coomassie Blue.

for Recombinant DNMT3A (278-432) proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant DNMT3A (278-432) proteinGuarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

 

Technical Data Sheet

Recombinant DNMT3A (278-432) protein
 

Data Thumbnails

Recombinant DNMT3A (278-432), GST-Tag gel.

DNMT3A (278-432) was run on a 12% SDS-PAGE gel and stained with Coomassie Blue.
(Click image to enlarge and see details).