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リコンビナントタンパク質・酵素

Recombinant BRPF1 (629-742) protein

Aliases: Bromodomain And PHD Finger Containing 1, Peregrin, BR140
商品コード: 31547 Format: 100 µg ¥73,000 Buy Now
商品コード: 31947 Format: 1 mg ¥537,000 Buy Now
Expressed In: E. coli Protein Species: Human

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for Recombinant BRPF1 (629-742) proteinContents

Recombinant BRPF1 (629-742), GST-Tag is suppled in 25 mM Tris pH 8.0, 300 mM NaCl, 5% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant BRPF1 (629-742) proteinBackground

Bromodomain and PHD  finger containing (BRPF1) protein is a component of the MOZ/MORF complex which has histone H3 acetyltransferase activity. The bromodomain of BRPF1 recognizes acetylated histone lysine residues and function as a ‘reader’ of these epigenetic histone marks to regulate chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. BRPF1 is known to positively regulate the transcription of RUNX1 and RUNX2.

for Recombinant BRPF1 (629-742) proteinApplication Notes

Recombinant BRPF1 (629-742), GST-Tag is suitable for use in binding assays, inhibitor screening, and selectivity profiling. 

for Recombinant BRPF1 (629-742) proteinProtein Details

The peptide corresponding to amino acids 629 - 742 that contains the bromodomain sequences of BRPF1 (accession number NP_004625.2) was expressed in E. coli cells and contains an N-terminal GST-Tag with a molecular weight of 39.4 kDa. BRPF1 (629-742), GST-Tag is >92% pure by SDS-PAGE.

 
Recombinant BRPF1 (629-742), GST-Tag gel.

Recombinant BRPF1 (629-742) Protein SDS-PAGE Gel.
BRPF1 (629-742) protein was run on a 12% SDS-PAGE gel and stained with Coomassie Blue.

HTRF assay for BRPF1 (629-742) protein activity.

HTRF assay for BRPF1 (629-742) protein activity.
3 µM histone peptide H4K5/8/12/16(4Ac) was incubated with BRPF1 (629-742), GST-Tag in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature.

HTRF assay for BRPF1 (629-742), GST-Tag activity

HTRF assay for BRPF1 (629-742) protein activity.
3 µM histone peptide H4K5/8/12/16(4Ac) was incubated with BRPF1 (629-742), GST-Tag in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature.

for Recombinant BRPF1 (629-742) proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant BRPF1 (629-742) proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant BRPF1 (629-742) protein
 

Data Thumbnails

Recombinant BRPF1 (629-742), GST-Tag gel.

BRPF1 (629-742), GST-Tag protein was run on a 12% SDS-PAGE gel and stained with Coomassie Blue.
(Click image to enlarge and see details).

HTRF assay for BRPF1 (629-742) protein activity.

3 µM histone peptide H4K5/8/12/16(4Ac) was incubated with BRPF1 (629-742), GST-Tagged protein in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-GST antibody was used to detect reaction products.
(Click image to enlarge and see details).

HTRF assay for BRPF1 (629-742), GST-Tag activity

3 µM histone peptide H4K5/8/12/16(4Ac) was incubated with BRPF1 (629-742), GST-Tag in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-GST antibody was used to detect reaction products.
(Click image to enlarge and see details).