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Recombinant KAT2A (GCN5) protein

商品コード: 31591 Format: 20 µg ¥73,000 Buy Now
Expressed In: Baculovirus Protein Species: Human

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for Recombinant KAT2A (GCN5) proteinContents

Recombinant KAT2A (GCN5) protein is supplied in 25 mM HEPES pH 7.5, 300 mM NaCl, 5% glycerol, 0.04% Triton X-100, 0.2 mM TCEP. Please refer to product insert for lot-specific concentration.

for Recombinant KAT2A (GCN5) proteinBackground

GCN5 (General Control Of Amino Acid Synthesis Protein 5, also known as KAT2A, Lysine Acetyltransferase 2A) is a histone acetyltransferase (HAT) that functions primarily as a transcriptional activator. It also acts as a repressor of NF-kappa-B by promoting ubiquitination of the NF-kappa-B subunit RELA in a HAT- independent manner. KAT2A (GCN5) has significant histone acetyltransferase activity with core histones, but not with nucleosome core particles. It also acetylates non-histone proteins, such as CEBPB. KAT2A (GCN5) is a core component of the ATAC complex, which is a complex with histone acetyltransferase activity on histones H3 and H4.

for Recombinant KAT2A (GCN5) proteinApplication Notes

Recombinant KAT2A (GCN5) protein is suitable for use in enzyme kinetics, inhibitor screening, and selectivity profiling.

for Recombinant KAT2A (GCN5) proteinProtein Details

Recombinant KAT2A (GCN5) protein was expressed in a Baculovirus expression system as the full length protein (accession number NP_066564.2) with an N-terminal FLAG-Tag. The molecular weight of the protein is 95.6 kDa.

 
Recombinant KAT2A (GCN5) protein, SDS-PAGE gel.

Recombinant KAT2A (GCN5) protein, SDS-PAGE gel
Recombinant KAT2A (GCN5) protein was run on an 8% SDS-PAGE gel and stained with Coomassie Blue.
MW: 95.6 kDa
Purity: > 85%

Recombinant KAT2A (GCN5) protein activity assay.

Recombinant KAT2A (GCN5) protein activity assay
50 µM H3 peptide was incubated with KAT2A (GCN5) protein in reaction buffer for 25 minutes at room temperature. The reaction was stopped and developed for 15 minutes in the dark at RT. Fluorescence was read with excitation at 380 nm and emission at 460 nm.
HAT Assay Kit (Fluorescent, Cat# 56100) was used for KAT2A (GCN5) activity detection.

Recombinant KAT2A (GCN5) protein activity assay.

Recombinant KAT2A (GCN5) protein activity assay
50 µM H3 peptide was incubated with KAT2A (GCN5) protein in reaction buffer for 25 minutes at room temperature. The reaction was stopped and developed for 15 minutes in the dark at RT. Fluorescence was read with excitation at 380 nm and emission at 460 nm.
HAT Assay Kit (Fluorescent, Cat# 56100) was used for KAT2A (GCN5) activity detection.

for Recombinant KAT2A (GCN5) proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant KAT2A (GCN5) proteinGuarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

 

Technical Data Sheet

Recombinant KAT2A (GCN5) protein
 

Data Thumbnails

Recombinant KAT2A (GCN5) protein, SDS-PAGE gel.

Recombinant KAT2A (GCN5) protein, SDS-PAGE gel.
(Click to enlarge and view details)

Recombinant KAT2A (GCN5) protein activity assay.

Recombinant KAT2A (GCN5) protein activity assay
(Click to enlarge and view details)

Recombinant KAT2A (GCN5) protein activity assay.

Recombinant KAT2A (GCN5) protein activity assay
(Click to enlarge and view details)