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Recombinant IDH1 protein

商品コード: 31610 Format: 100 µg ¥73,000 Buy Now
Expressed In: E. coli Protein Species: Human

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for Recombinant IDH1 proteinContents

Recombinant IDH1 protein is supplied in 25 mM Tris pH 8.0, 300 mM NaCl, 5% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant IDH1 proteinBackground

IDH1 (Isocitrate Dehydrogenase (NADP(+)) 1, Cytosolic, also known as HEL-216, HEL-S-26, IDCD, IDH, IDP, IDPC, PICD) is a member of isocitrate dehydrogenases, which catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. Isocitrate dehydrogenases belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer.
IDH is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production.

for Recombinant IDH1 proteinApplication Notes

This protein is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

for Recombinant IDH1 proteinProtein Details

Recombinant IDH1 protein was expressed in E. coli cells as the full length protein (accession number AAH93020.1) with a C-terminal 6×His-Tag. The molecular weight of the protein is 47.7 kDa.

 
Recombinant IDH1 protein, SDS-PAGE gel.

Recombinant IDH1 protein, SDS-PAGE gel.
Recombinant IDH1 protein was run on a 10% SDS-PAGE gel and stained with Coomassie Blue.
MW: 47.7 kDa
Purity: > 90%

Recombinant IDH1 protein activity assay.

Recombinant IDH1 protein activity assay.
200 µM DL-isocitric acid trisodium and 200 µM NADP+ were incubated with IDH1 protein in reaction buffer including 50 mM Tris-HCl pH 7.4, 100 mM MgCl2 for 10 minutes at room temperature. The generation of product NADPH was monitored by measuring absorbance at 340 nm.

Recombinant IDH1 protein activity assay.

Recombinant IDH1 protein activity assay.
200 µM DL-isocitric acid trisodium and 200 µM NADP+ were incubated with IDH1 protein in reaction buffer including 50 mM Tris-HCl pH 7.4, 100 mM MgCl2 for 10 minutes at room temperature. The generation of product NADPH was monitored by measuring absorbance at 340 nm.

for Recombinant IDH1 proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant IDH1 proteinGuarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

 

Technical Data Sheet

Recombinant IDH1 protein
 

Data Thumbnails

Recombinant IDH1 protein, SDS-PAGE gel.

Recombinant IDH1 protein, SDS-PAGE gel.
(Click to enlarge and view details).

Recombinant IDH1 protein activity assay.

Recombinant IDH1 protein activity assay.
(Click to enlarge and view details).

Recombinant IDH1 protein activity assay.

Recombinant IDH1 protein activity assay.
(Click to enlarge and view details).