Active Motif,
Tools to analyze nuclear function,
Your CartYour Cart 0 items
細胞・組織・酸抽出物

HeLa nuclear extract (2 hr serum response)

商品コード: 36104 Format: 200 µg ¥31,000 Buy Now

for HeLa nuclear extract (2 hr serum response)Contents

200 µg of HeLa nuclear extract (2 hr serum response) at 4.3 µg/µl.

for HeLa nuclear extract (2 hr serum response)Background

HeLa nuclear extract (2 hr serum response) was prepared from a cell culture of the HeLa human epithelial carcinoma cell line. The HeLa cell line was originally derived from cells obtained in 1951 from Henrietta Lacks, a cervical cancer patient who eventually died from her cancer. The HeLa cell line is one of the oldest and most commonly utilized immortal cell lines in scientific research. These cells are highly prolific and easily sustainable, allowing for flexibility in their application in various fields of scientific research, including cancer biology, virology, and human disease. To date, over 60,000 publications across various disciplines have referenced the use of HeLa cells.

The addition of serum to HeLa cells after serum starvation stimulates various biological processes associated with induction of gene expression, differentiation, proliferation, and apoptosis. Therefore, serum-stimulated cells are commonly utilized in research to analyze activation of transcription of early response genes. It has been shown that serum stimulation leads to activation of several key transcription factors, including activator protein 1 (AP-1) family and Sp1 that regulate the expression of early response genes.

for HeLa nuclear extract (2 hr serum response)Application Notes

HeLa nuclear extract (2 hr serum response) is specifically recommended for analysis of 1) AP-1 and Sp1 mediated gene expression, 2) differentiation, 3) proliferation, and 4) cell survival.

for HeLa nuclear extract (2 hr serum response)Extract Origin

Human Cervix Adenocarcinoma

for HeLa nuclear extract (2 hr serum response)Extract Composition

HeLa nuclear extract (2 hr serum response) is supplied in Dilution Buffer (20 mM Hepes (pH 7.9), 100 mM KCl, 1 mM MgCl2, 20% glycerol, 0.5 mM PMSF and 0.5 mM DTT). Cells were cultured for 24 hours in low serum (0.5%) conditions and then serum-stimulated (10%) for 2 hours immediately prior to harvesting.

for HeLa nuclear extract (2 hr serum response)Quality Control

Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).

for HeLa nuclear extract (2 hr serum response)Storage

To ensure stability, extracts should be stored at -80°C.

We recommend aliquoting the extracts into single-use fractions and then storing them at -80°C. This eliminates repeated freeze/thaw cycles.

for HeLa nuclear extract (2 hr serum response)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

HeLa nuclear extract (2 hr serum response)