AP-2 antibody (pAb)

RRID: AB_2793148
Aliases: TFAP2A, BOFS, AP2TF, TFAP2, AP-2alpha
Catalog No: 39001 Format: 100 µg Discontinued

Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
50 and 40 kDa
Reactivity:
Human

Applications

ChIP Validated Western Blot Validated Immunohistochemistry Validated Electrophoretic Mobility Shift Assay Validated

Application Notes

This product has been discontinued:

Applications Validated by Active Motif:
WB*: 1:500 - 1:1,000

*For optimal results, primary antibody incubations should be performed overnight at 4°C. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background. Individual optimization may be required.

Published Applications

The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:

ChIP
IHC(P)
EMSA

Immunogen

This AP-2 antibody was raised against a synthetic peptide corresponding to amino acid residues 120-134 of human AP-2.

Buffer

PBS containing 0.2% gelatin, 0.01% thimersol and 0.1% sodium azide. Thimersol and sodium azide are highly toxic.

 

AP-2 pAb tested by Western blot.
Detection of AP-2 by Western blot analysis. Activator protein 2 (AP-2) can be detected in JEG-3 nuclear extract using AP-2 rabbit polyclonal antibody at a 1:1,000 dilution.

Background

AP-2 (activator protein 2 or AP2) transcription factors activate transcription and regulate gene expression during embryogenesis. There are four different isoforms of AP-2 (AP-2α, AP-2β, AP-2γ and AP-2δ). The family members share a common structure in which the N-terminal region is involved with transcriptional activation and the C-terminal region is involved in DNA binding.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with AP-2 antibody (pAb).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting