c-Myc antibody (pAb) (65 kDa form)

for c-Myc antibody (pAb) (65 kDa form)Application Notes

Validated Applications:
WB: 1:500 - 1:1,000 dilution

Published Applications: see references

For optimal results, primary antibody incubations should be performed overnight at 37°C. Individual optimization may be required.

for c-Myc antibody (pAb) (65 kDa form)Immunogen

This c-Myc antibody was raised against a synthetic peptide corresponding to amino acid residues 9-22 of human c-Myc; it recognizes a 65 kDa form of c-Myc.

for c-Myc antibody (pAb) (65 kDa form)Buffer

PBS containing 0.2% gelatin, 0.01% thimersol and 0.1% sodium azide. Thimersol and sodium azide are highly toxic.

for c-Myc antibody (pAb) (65 kDa form)References

for c-Myc antibody (pAb) (65 kDa form)Background

c-Myc is a sequence specific DNA binding transcription factor that regulates gene expression, especially genes involved in cell division. Myc family members contain a basic Helix-Loop-Helix leucine zipper (bHLHZ) domain. Myc family proteins associated with thousands of genes, especially those involved in cell growth and proliferation. Myc serves as an integrator of signal transduction, linking extracellular signals to an acute cellular growth response. Mutations and overexpression of c-Myc are associated with cell proliferation and cancer.

for c-Myc antibody (pAb) (65 kDa form)Positive Control

Active Motif's Jurkat nuclear extract (Catalog No. 36014) can be used as a positive control for c-Myc antibody (65 kDa form).

for c-Myc antibody (pAb) (65 kDa form)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for c-Myc antibody (pAb) (65 kDa form)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.