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Antibodies

GATA-1 antibody (pAb)

Catalog No: 39025 Format: 100 µl $340 Buy Now
Antibody Type:Polyclonal
Host:Rabbit
Isotype:Serum
Purification:None
Applications:ChIP, WB
Reactivity:Human, Mouse, Rat
Molecular Weight:47 kDa

Applications Table

Western Blot Chromatin IP
1:1,000 Published.
See References below.

for GATA-1 antibody (pAb)Background

GATA-1 (also designated Globin transcription factor 1, GATA binding protein 1 and GATA1) is a member of the GATA Family of transcription factors. GATA-1 is involved in erythroid development by regulating the switch of fetal hemoglobin to adult hemoglobin.

for GATA-1 antibody (pAb)Immunogen

This GATA-1 antibody was raised against a synthetic peptide corresponding to amino acid residues 66-78 of human GATA-1.

for GATA-1 antibody (pAb)Application Notes

This GATA-1 antibody has been validated for use in Western blotting (1:1,000 dilution). For optimal results, primary antibody incubations should be performed overnight at 4°C. The addition of 0.1% Tween 20 to all Blotto solutions may reduce background. Individual optimization may be required.

This antibody has also been validated in chromatin immunoprecipitation (ChIP). See reference for details.

for GATA-1 antibody (pAb)Buffer

PBS containing 0.2% gelatin, 0.01% thimersol and 0.1% sodium azide.

for GATA-1 antibody (pAb)Positive Control

Active Motif's K-562 nuclear extract (Catalog No. 36015) can be used as a positive control for GATA-1 antibody.

Chromatin IP Assays

Active Motif has a variety of Chromatin Immunoprecipitation Kits for every need.
Please visit our Chromatin IP Page to learn about ChIP products designed for use with GATA-1 antibody (pAb).

for GATA-1 antibody (pAb)References

The use of this antibody is described in the following reference:
ChIP: de Vooght, KM et al ( 2008) Gene 15;409(1-2):83-91

for GATA-1 antibody (pAb)Storage

Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for GATA-1 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FACS = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • WB = Western Blotting