NF-1A antibody (pAb)

for NF-1A antibody (pAb)Background

NF-1A (NF-1, Nuclear factor-1) is a ubiquitous 47 kDa dimeric DNA-binding protein whose recognition sequence, TGG(C/A)N(5)GCCAA, is found in the genomes of a number of DNA viruses. The same sequence occurs frequently in the human genome. The NF-1 protein stimulates initiation of adenovirus DNA replication in vitro and is capable of stimulating the transcription of genes in cooperation with other factors, such as the estrogen receptor. The Nuclear factor-1 (NF-1) family of transcription factors are encoded by the genes NF-1A, NF-1B2, NF-1C and NF-1X and feature a highly conserved N-terminal DNA binding region and a variable C-terminal transcriptional activation or repression domain. NF-1 binding controls the expression of many target genes, including insulin, TGF-β, nucleotide, steroid hormone and vitamin synthetic genes, TNFα and FSH. NF-1 transcripts can be differentially spliced and the resultant protein variants homo- and heterodimerize, allowing for multiple potential transcriptional activation and repression activities.

for NF-1A antibody (pAb)Immunogen

This NF-1A antibody was raised against a synthetic peptide corresponding to amino acid residues 478-492 of human NF-1A.

for NF-1A antibody (pAb)Application Notes

This NF-1A antibody has been validated for use in Western blotting (1:500 - 1:1,000 dilution). For optimal results, primary antibody incubations should be performed at 37°C. Individual optimization may be required.

for NF-1A antibody (pAb)Buffer

PBS containing 0.2% gelatin, 0.01% thimersol and 0.1% sodium azide. Thimersol and sodium azide are highly toxic.

for NF-1A antibody (pAb)Positive Control

Active Motif's Rat Liver nuclear extract (Catalog No. 36024) can be used as a positive control for NF-1A antibody.

for NF-1A antibody (pAb)Storage

Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for NF-1A antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.