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Histone H3S28ph antibody (mAb)
| RRID: AB_2793154 |
| Clone: HTA28 |
| 商品コード: 39098 | Format: 100 µl | ¥66,800 | Buy |
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Applications
Published Applications
The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:
IHC(P)
View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.
Immunogen
This Histone H3 phospho Ser28 antibody was raised in rat against a peptide corresponding to amino acid residues 23-35 (phospho Ser 28) of human Histone H3.
Buffer
Purified IgG in 0.1 M Tris-glycine (pH 7.4), 150 mM NaCl and 0.05% sodium azide. Sodium azide is highly toxic. For your convenience, an ascites version (Catalog No. 61325) of this antibody is also available.
View our Guide to Histone Modifications and Biological Function.
Histone H3 phospho Ser28 antibody tested by immunofluorescence.
Top: HeLa cells stained with Histone H3 phospho Ser28 antibody at a 1:1,000 dilution.
Middle: Same cells stained with DAPI.
Bottom: Merge of both images.
Histone H3 phospho Ser28 antibody specificity tested by peptide array analysis.
Peptide array analysis was used to confirm the specificity of this antibody for its intended modification. Histone H3 phospho Ser28 antibody was applied at a dilution of 1:3,000 to Active Motif's MODified™ Histone Peptide Array (Catalog No. 13001). The arrays were scanned with ArrayAnalysis Software 7 and the results plotted. Specificity data is shown for the most reactive peptides and those related to the immunogen. Recognition of the Histone H3 phospho Ser28 peptides by the antibody is inhibited by Lys27 monomethylation and blocked by Arg26 dimethylation and a citrulline at position 26.
Array Data File
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
- WB = Western Blotting
