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ASH2 antibody (pAb)
| Catalog No: 39099 | Format: 200 µl | $340 | Buy Now |
| Catalog No: 39100 | Format: 10 µl | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP | IF | IP |
|---|---|---|---|
| 1:500 - 1:2,000 | — | 1:500 | 10 µl per IP |
for ASH2 antibody (pAb)Background
ASH2 (absent, small or homeotic discs 2) is a trithorax protein and is a component of different protein complexes, including i.e. mixed-lineage leukemia (MLL) oncoproteins, which contain histone methyltransferase activity. These complexes trimethylate the lysine 4 of histone H3. Histone H3 trimethyl Lys4 is one of histone modifications that are part of elaborate mechanisms that evolved to control the structure of the chromatin.
for ASH2 antibody (pAb)Immunogen
This ASH2 antibody was raised against full-length recombinant human ASH2 protein.
for ASH2 antibody (pAb)Application Notes
This ASH2 antibody has been validated for use in Western blotting (1:500 - 1:2,000 dilution), immunofluorescence (1:500 dilution) and immunoprecipitation (10 µl per IP).
for ASH2 antibody (pAb)Buffer
Rabbit serum containing 30% glycerol and 0.035% sodium azide.
for ASH2 antibody (pAb)Positive Control
Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for ASH2 antibody.
Compatible Secondary Antibodies
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with ASH2 antibody (pAb).
for ASH2 antibody (pAb)References
This antibody was used in Sims et al (2007) Mol Cell 28:665.
ASH2 pAb tested by Western blot.
ASH2 detection by Western blot. The analysis of ASH2 was performed using HeLa nuclear extract and ASH2 pAb at a 1:2,000 dilution.
for ASH2 antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for ASH2 antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
