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Histone H2AK9ac antibody (pAb)
| RRID: AB_2793158 |
| 商品コード: 39109 | Format: 200 µl | ¥66,800 | Buy |
| 商品コード: 39110 | Format: 10 µl | ¥18,800 | Buy |
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Applications
Published Applications
The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:
WB
View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.
Immunogen
This Histone H2A acetyl Lys9 antibody was raised against a peptide including acetyl-lysine 9 of human histone H2A.
Buffer
Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
View our Guide to Histone Modifications and Biological Function.
Histone H2A acetyl Lys9 pAb tested by Western blot.
Western blot probed with Histone H2A acetyl Lys9 polyclonal antibody (1:5,000 dilution).
Lane 1: 20 ng recombinant histone H2A.
Lane 2: 5 µg acid extract of HeLa cells.
Lane 3: 5 µg acid extract of HeLa cells treated with sodium butyrate.
Specificity data:
Dot blot analysis was used to confirm the specificity of Histone H2A acetyl Lys9 pAb for acetyl Lys9 of Histone H2A. Modified and unmodified peptides were spotted onto PVDF and probed with the antibody at a 1:5,000 dilution. The amount of peptide spotted (in picomoles) is indicated next to each row.
Lane 1: Peptide acetylated at Lys5 of H2A.
Lane 2: Unmodified Lys5 peptide.
Lane 3: Peptide acetylated at Lys9 of H2A.
Lane 4: Unmodified Lys9 peptide.
Detection of H2AK9ac by immunofluorescence.
U2OS cells were stained with H2AK9ac antibody at a dilution of 1:500. Left panel: DAPI. Middle panel: H2AK9ac antibody staining. Right panel: merge.
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
- WB = Western Blotting
