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Histone H3K27ac antibody (pAb)

商品コード: 39133 Format: 100 µg ¥69,000 Buy Now
商品コード: 39134 Format: 10 µg ¥20,000 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Protein A Chromatography
Host:Rabbit
Molecular Weight:17 kDa
Reactivity:Budding Yeast, Human, Other (Wide Range)

Applications

ChIP Validated ChIP-Seq Validated Western Blot Validated Immunofluorescence Validated Dot Blot Validated Immunocytochemistry Validated

for Histone H3 acetyl Lys27 antibody (pAb)Application Notes


Validated Applications:
ChIP: 5 µl per ChIP
ChIP-Seq: 5 µl each
ICC/IF: 1 - 5 µg/ml dilution
WB: 0.5 - 2 µg/ml dilution

modENCODE validation. This antibody was validated for ChIP-Seq in this study (see references).

NGS-QC® certification. This antibody has been processed by the NGS-QC® generator. For additional details, click here..

Published Applications:
ChIP-Seq
ChIP
WB
IF


See references for more information. Individual optimization may be required.

for Histone H3 acetyl Lys27 antibody (pAb)Immunogen

This Histone H3 acetyl Lys27 antibody was raised against a peptide including acetyl-lysine 27 of histone H3.

for Histone H3 acetyl Lys27 antibody (pAb)Buffer

Purified IgG in 10 mM sodium phosphate (pH 7.5), 150 mM NaCl, 30% glycerol, 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, an unpurified serum version (Catalog No. 39135) of this antibody is also available.

for Histone H3 acetyl Lys27 antibody (pAb)References

 
Histone H3K27ac antibody (pAb) tested by ChIP-Seq.

Histone H3 acetyl Lys27 antibody (pAb) tested by ChIP-chip.
ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with chromatin from a human hepatocyte cell line. ChIP DNA was amplified by WGA, labeled and hybridized to a human tiling array. The image shows H3K27Ac binding sites across a 1MB region on chromosome 11.

Histone H3K27ac antibody (pAb) tested by ChIP.

Histone H3 acetyl Lys27 antibody tested by ChIP qPCR analysis.
ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with chromatin from a human hepatocyte cell line. qPCR was performed using a negative control primer set (Untr20) and three positive control primer sets that amplify known H3K27Ac sites.

Histone H3K27ac antibody (pAb) tested by immunofluorescence.

Histone H3 acetyl Lys27 antibody tested by immunofluorescence.
Staining of HEK293 cells with Histone H3 acetyl Lys27 antibody (1 µg/ml dilution, top panel) and DAPI (bottom panel).

Histone H3K27ac antibody (pAb) tested by Western blot.

Histone H3 acetyl Lys27 antibody tested by Western blot.
HeLa acid extract probed with Histone H3 acetyl Lys27 polyclonal antibody (0.5 µg/ml).
    Lane 1: No treatment.
    Lane 2: Cells treated with sodium butyrate.

Histone H3K27ac antibody (pAb) tested by dot blot analysis.

Histone H3 acetyl Lys27 antibody tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H3 acetyl Lys27 antibody for acetyl Lys27 histone H3. Acetylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at the indicated concentration for each panel. The amount of peptide (picomoles) spotted is indicated next to each row.
Top Panel (antibody at 0.2 µg/ml)- Lane 1: Unmodified histone H3 Lys14. Lane 2: Ac-Lys14. Lane 3: Ac-Lys9. Lane 4: Ac-Lys18. Lane 5: Ac-Lys23. Lane 6: Ac-Lys27. Lane 7: Ac-Lys37. Lane 8: Ac-Lys36. Lane 9: Histone H2A Ac-Lys5. Lane 10: Histone H2A Ac-Lys9.
Bottom Panel (antibody at 1 µg/ml)- Lane 1: Histone H3 Ac-Lys9. Lane 2: Unmodified Lys9. Lane 3: Ac-Lys14. Lane 4: Unmodified Lys14. Lane 5: Ac-Lys18. Lane 6: Unmodified Lys18. Lane 7: Ac-Lys23. Lane 8: Unmodified Lys23. Lane 9: Ac-Lys27. Lane 10: Unmodified Lys27.

for Histone H3 acetyl Lys27 antibody (pAb)Background

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.

Lysine 27 of histone H3 can also be mono-, di- or trimethylated by different histone methyltransferases, such as EZH2 or NSD3. While histone methylation can be associated with transcriptional activation or repression, methylation of Lysine 27 of histone H3 is mainly associated with transcriptional repression.

for Histone H3 acetyl Lys27 antibody (pAb)Positive Control

Active Motif's HeLa acid extract (Sodium butyrate treated) (Catalog No. 36202) can be used as a positive control for Histone H3 acetyl Lys27 antibody.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H3K27ac antibody (pAb).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Histone H3K27ac antibody (pAb).

for Histone H3 acetyl Lys27 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Histone H3 acetyl Lys27 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Histone H3K27ac antibody (pAb)
 

Data Thumbnails

Histone H3K27ac antibody (pAb) tested by ChIP-Seq.

ChIP-Seq of Histone H3K27ac pAb.
(Click image to enlarge and see details.)

Histone H3K27ac antibody (pAb) tested by ChIP.

ChIP of Histone H3K27ac pAb.
(Click image to enlarge and see details.)

Histone H3K27ac antibody (pAb) tested by immunofluorescence.

Immunofluorescence stain of Histone H3K27ac pAb.
(Click image to enlarge and see details.)

Histone H3K27ac antibody (pAb) tested by Western blot.

Western blot of Histone H3K27ac pAb.
(Click image to enlarge and see details.)

Histone H3K27ac antibody (pAb) tested by dot blot analysis.

Dot blot of Histone H3K27ac pAb.
(Click image to enlarge and see details.)