Histone H3 acetyl Lys27 antibody (pAb)

for Histone H3 acetyl Lys27 antibody (pAb)Background

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.

Lysine N-ε-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Histone acetylation is often associated with transcriptional activation.

Lysine 27 of histone H3 can also be mono-, di- or trimethylated by different histone methyltransferases, such as EZH2 or NSD3. While histone methylation can be associated with transcriptional activation or repression, methylation of Lysine 27 of histone H3 is mainly associated with transcriptional repression.

for Histone H3 acetyl Lys27 antibody (pAb)Immunogen

This histone H3 acetyl Lys27 antibody was raised against a peptide including acetyl-lysine 27 of histone H3.

for Histone H3 acetyl Lys27 antibody (pAb)Application Notes

This Histone H3 acetyl Lys27 antibody (H3K27ac) has been validated for use in chromatin IP (5 µl per ChIP), ChIP-Seq & ChIP-chip (5 µl each), Western blotting (0.5 - 2 µg/ml) and immunofluorescence (1 - 5 µg/ml). Specificity for acetyl-lysine 27 was confirmed by dot blot with no cross-reactivity to non-modified histone H3 peptide or peptides acetylated at lysines 9, 14, 18 or 23. Because this modification is conserved from yeast to human, a wide range of species reactivity is expected.

The modENCODE and NIH Roadmap Epigenomics Mapping Consortiums have implemented rigorous standardization criteria for all assays and reagents to be used. As part of this initiative, antibody specificity testing and the ability of the antibodies to work in ChIP-Seq were assessed in a large-scale study. This Histone H3 acetyl Lys27 antibody was validated for ChIP-Seq in the study (see References).

for Histone H3 acetyl Lys27 antibody (pAb)Buffer

10 mM sodium phosphate pH 7.5, 150 mM NaCl, 30% glycerol, 0.035% sodium azide.

for Histone H3 acetyl Lys27 antibody (pAb)Positive Control

Active Motif's HeLa acid extract (Sodium butyrate treated) (Catalog No. 36202) can be used as a positive control for Histone H3 acetyl Lys27 antibody.

for Histone H3 acetyl Lys27 antibody (pAb)References

This antibody has been used as described below:

for Histone H3 acetyl Lys27 antibody (pAb)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Histone H3 acetyl Lys27 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.