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Histone H3 phospho Ser10,28 antibody (pAb)
| Catalog No: 39147 | Format: 200 µl | $340 | Buy Now |
| Catalog No: 39148 | Format: 10 µl | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP | Immunofluorescence |
|---|---|---|
| 1:1,000 - 1:10,000 | 10 µl per ChIP | 1:500 - 1:2,000 |
for Histone H3 phospho Ser10,28 antibody (pAb)Background
Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.
Ser10 phosphorylation and Ser28 phosphorylation in the tail of H3 have very similar kinetics. Both phosphorylations occur early in mitosis when chromosomes begin to condense, and during premature chromosome condensation induced in S-phase cells. These phosphorylated serines are excellent mitotic markers.
for Histone H3 phospho Ser10,28 antibody (pAb)Immunogen
This This Histone H3 phospho Ser10,28 antibody was raised against a peptide including phosphorylated serine 28 of human Histone H3.
for Histone H3 phospho Ser10,28 antibody (pAb)Application Notes
This Histone H3 phospho Ser10,28 antibody (H3S10Ph) has been validated for use in Western blotting (1:1,000 - 1:10,000 dilution), immunofluorescence (1:500 - 1:2,000 dilution) and chromatin IP (10 µl per ChIP). Its specificity was determined by dot blots with a non-modified histone H3 peptide and peptides phosphorylated at serines 10 or 28. Because this modification is conserved from yeast to human, a wide range of reactivity is expected.
for Histone H3 phospho Ser10,28 antibody (pAb)Buffer
Rabbit serum containing 30% glycerol and 0.035% sodium azide.
for Histone H3 phospho Ser10,28 antibody (pAb)Positive Control
Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for Histone H3 phospho Ser10,28 antibody.
Chromatin IP Assays
Active Motif has a variety of Chromatin Immunoprecipitation Kits for every need.
Please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H3 phospho Ser10,28 antibody (pAb).
Compatible Secondary Antibodies
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Histone H3 phospho Ser10,28 antibody (pAb).
Histone H3 phospho Ser10,28 antibody tested by Western blot.
HeLa acid extract probed with Catalog No. 39147 (1:5,000 dilution).
Lane 1: No treatment.
Lane 2: Cells treated with colcemid.
Histone H3 phospho Ser10,28 antibody tested by immunofluorescence.
HeLa cells stained with Histone H3 phospho Ser10,28 antibody at a 1:1,000 dilution.
Top panel: Histone H3 phospho Ser10,28 antibody.
Middle: DAPI.
Bottom: Merge of both images.
Histone H3 phospho Ser10,28 antibody tested by ChIP analysis.
Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and HeLa Chromatin (1.5 x 10^6 cell equivalents per ChIP) using 10 µl of Histone H3 phospho Ser10, 28 antibody or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the GAPDH gene promoter. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.
for Histone H3 phospho Ser10,28 antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Histone H3 phospho Ser10,28 antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
