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Histone H3 phospho Thr3 antibody (pAb)
| Catalog No: 39153 | Format: 200 µl | $340 | Buy Now |
| Catalog No: 39154 | Format: 10 µl | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP |
|---|---|
| 1:500 - 1:2,000 | — |
for Histone H3 phospho Thr3 antibody (pAb)Background
Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.
In vivo, phosphorylation of histone H3 at threonine 3 begins at early prophase in the vicinity of the nuclear envelope, spreads to the pericentromeric chromatin during prometaphase and is fully reversed by late anaphase, during mitosis.
for Histone H3 phospho Thr3 antibody (pAb)Immunogen
This antibody was raised against a peptide including phospho-threonine 3 of histone H3.
for Histone H3 phospho Thr3 antibody (pAb)Application Notes
This Histone H3 phospho Thr3 antibody (H3T3Ph) has been validated for use in Western blotting of cells arrested in mitosis (1:500 - 1:2,000 dilution). Specificity for phospho-threonine 3 was confirmed by dot blot and peptide competition. Treatment of cells with colcemid strongly increases detection by Western blot. No recognition of recombinant histone H3 is observed by Western blot. Because this modification is highly conserved, a wide range of reactivity is expected.
for Histone H3 phospho Thr3 antibody (pAb)Buffer
Rabbit serum containing 30% glycerol and 0.035% sodium azide.
for Histone H3 phospho Thr3 antibody (pAb)Positive Control
Active Motif's HeLa acid extract (Paclitaxel treated) (Catalog No. 36201) can be used as a positive control for Histone H3 phospho Thr3 antibody.
Histone H3 phospho Thr3 pAb tested by Western blot.
Western blot probed with Histone H3 phospho Thr3 pAb (1:500 dilution).
Lane 1: 20 µg acid extract of HeLa cells.
Lane 2: 20 µg acid extract of HeLa cells treated with colcemid.
Histone H3 phospho Thr3 pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H3 phospho Thr3 pAb for phospho-Thr3 of histone H3. Decreasing amounts of peptides corresponding to regions around major sites of histone H3 threonine phosphorylation were spotted onto PVDF and probed with the antibody at a dilution of 1:2,000.
Lane 1: Peptide phosphorylated at threonine 3.
Lane 2: Unmodified threonine 3 peptide.
Lane 3: Peptide phosphorylated at threonine 11.
Lane 4: Unmodified threonine 11 peptide.
Lane 5: Peptide phosphorylated at threonine 45.
Lane 6: Unmodified threonine 45 peptide.
for Histone H3 phospho Thr3 antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Histone H3 phospho Thr3 antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
