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Histone H4K5ac antibody (pAb)

商品コード: 39169 Format: 200 µl ¥67,000 Buy Now
商品コード: 39170 Format: 10 µl ¥19,000 Buy Now

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Antibody Type:Polyclonal
Isotype:Serum
Purification:None
Host:Rabbit
Molecular Weight:8 kDa
Reactivity:Human, Other (Wide Range)

Applications

ChIP Validated Western Blot Validated Immunofluorescence Validated Dot Blot Validated Immunocytochemistry Validated

for Histone H4 acetyl Lys5 antibody (pAb)Application Notes


Validated Applications:
ChIP: 3 - 10 µl per ChIP
ICC/IF: 1:500 - 1:1,000 dilution
WB: 1:1,000 - 1:5,000 dilution

Published Applications:
ChIP
WB

for Histone H4 acetyl Lys5 antibody (pAb)Immunogen

This Histone H4 acetyl Lys5 antibody was raised against a peptide including acetyl-lysine 5 of histone H4.

for Histone H4 acetyl Lys5 antibody (pAb)Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for Histone H4 acetyl Lys5 antibody (pAb)References

 
Histone H4K5ac antibody (pAb) tested by ChIP.

Histone H4 acetyl Lys5 antibody tested by ChIP analysis.
Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) using 10 µl of Histone H4 acetyl Lys5 antibody or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the indicated gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.

Histone H4K5ac antibody (pAb) tested by ChIP.

Histone H4 acetyl Lys5 antibody tested by ChIP analysis.
Chromatin IP performed on HeLa cell chromatin using 39169. PCR was performed using primers specific for the promoter region of the human GAPDH gene. Lane 1: negative IgG control. Lane 2: ChIP using 10 ul of 39169. Lane 3: Input DNA control.

Histone H4K5ac antibody (pAb) tested by immunofluorescence.

Histone H4 acetyl Lys5 antibody tested by immunofluorescence.
Staining of HeLa cells with Histone H4 acetyl Lys5 antibody (1:500 dilution, top panel) and DAPI (bottom panel).

Histone H4K5ac antibody (pAb) tested by Western blot.

Histone H4 acetyl Lys5 antibody tested by Western blot.
HeLa acid extract probed with Histone H4 acetyl Lys5 polyclonal antibody (1:1,000 dilution).
    Lane 1: No treatment.
    Lane 2: Cells treated with sodium butyrate.

Histone H4K5ac antibody (pAb) tested by dot blot analysis.

Histone H4 acetyl Lys5 antibody specificity data.
Top Panel – Dot blot analysis was used to confirm the specificity of Histone H4 acetyl Lys5 antibody for H4 acetyl lysine 5. Peptides corresponding to regions around major sites of histone H4 acetylation were spotted onto PVDF and probed at a dilution of 1:1,000. The amount of peptide (in picomoles) spotted is indicated next to each row. Lane 1: acetyl-Lys5 peptide. Lane 2: unmodified Lys5 peptide. Lane 3: acetyl-Lys8 peptide. Lane 4: unmodified Lys8 peptide. Lane 5: acetyl-Lys12 peptide. Lane 6: unmodified Lys12 peptide. Lane 7: acetyl-Lys16 peptide. Lane 8: unmodified Lys16 peptide.
Bottom Panel – Peptide inhibition analysis. A Western blot was performed on butyrate-treated HeLa cell acid extract (5 µl per lane) at a dilution of 1:2,000. Different peptides were incubated with the antibody during the blotting procedure to determine if the recognition was blocked by a specific modification. Lane 1: no peptide. Lane 2: acetyl-Lys5 peptide. Lane 3: acetyl-Lys8 peptide. Lane 4: acetyl-Lys12 peptide. Lane 5: acetyl-Lys16 peptide.

for Histone H4 acetyl Lys5 antibody (pAb)Background

Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.

Lysine N-ε-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. H4 molecules acetylated at Lys5 or Lys8 are distributed in overlapping, but non-identical, islands throughout the euchromatic chromosome arms.

for Histone H4 acetyl Lys5 antibody (pAb)Positive Control

Active Motif's HeLa acid extract (Sodium butyrate treated) (Catalog No. 36202) can be used as a positive control for Histone H4 acetyl Lys5 antibody.

for Histone H4 acetyl Lys5 antibody (pAb)qPCR Primers

Active Motif's Human Positive Control Primer Set ACTB-2 (Cat. No. 71005) and Human Negative Control Primer Set 1 (Cat. No. 71001) have been validated for performing qPCR and endpoint PCR when this antibody has been used for ChIP on human samples. For ChIP experiments with this antibody on mouse samples, the Mouse Positive Control Primer Set Actb-2 (Cat. No. 71017) and Mouse Negative Control Primer Set 1 (Cat. No. 71011) have been validated for both qPCR and endpoint PCR.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H4K5ac antibody (pAb).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Histone H4K5ac antibody (pAb).

for Histone H4 acetyl Lys5 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Histone H4 acetyl Lys5 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Histone H4K5ac antibody (pAb)
 

Data Thumbnails

Histone H4K5ac antibody (pAb) tested by ChIP.

ChIP of Histone H4K5ac pAb.
(Click image to enlarge and see details.)

Histone H4K5ac antibody (pAb) tested by ChIP.

ChIP of Histone H4K5ac pAb.
(Click image to enlarge and see details.)

Histone H4K5ac antibody (pAb) tested by immunofluorescence.

Immunofluorescence stain of Histone H4K5ac pAb.
(Click image to enlarge and see details.)

Histone H4K5ac antibody (pAb) tested by Western blot.

Western blot of Histone H4K5ac pAb.
(Click image to enlarge and see details.)

Histone H4K5ac antibody (pAb) tested by dot blot analysis.

Dot blot of Histone H4K5ac pAb.
(Click image to enlarge and see details.)