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LSD1 antibody (pAb)
| Catalog No: 39186 | Format: 200 µl | $340 | Buy Now |
| Catalog No: 39187 | Format: 10 µl | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP | ChIP-Seq / ChIP-chip |
IP |
|---|---|---|---|
| 1:500 - 1:2,000 | 5 - 10 µl per ChIP | 5 µl per ChIP-Seq 5 µl per ChIP-chip |
10 µl per IP |
for LSD1 antibody (pAb)Background
LSD1 (Lysine-specific demethylase 1, also designated KDM1) was identified as a subunit of different complexes (such as CtBP, Co-REST, NRD or BRAF35). LSD1 is involved in transcriptional repression of genes through the demethylation of Histone H3 monomethyl Lys4 and Histone H3 dimethyl Lys4, a methylation site associated with transcriptional activation. LSD1 is also involved in transcriptional activation through the demethylation of Histone H3 monomethyl Lys9 and Histone H3 dimethyl Lys9, a modification often associated with transcriptional repression. LSD1 is also able to demethylate Lys370me1 and Lys370me2 in the regulatory domain of the tumor suppressor p53. LSD1 is a flavin-dependent amine oxidase.
for LSD1 antibody (pAb)Immunogen
This LSD1 antibody was raised against full-length recombinant human LSD1 protein.
for LSD1 antibody (pAb)Application Notes
This LSD1 antibody has been validated for use in chromatin IP (5 - 10 µl per ChIP), ChIP-Seq & ChIP-chip (5 µl each), Western blotting (1:500 - 1:2,000 dilution) and immunoprecipitation (10 µl per IP).
for LSD1 antibody (pAb)Buffer
Rabbit serum containing 30% glycerol and 0.035% sodium azide.
for LSD1 antibody (pAb)Positive Control
Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for LSD1 antibody.
Chromatin IP Assays
Active Motif has a variety of Chromatin Immunoprecipitation Kits for every need.
Please visit our Chromatin IP Page to learn about ChIP products designed for use with LSD1 antibody (pAb).
LSD1 pAb tested by Western blot.
Nuclear extract of HeLa cells (20 µg) was probed with LSD1 pAb (1:2,000 dilution).
LSD1 pAb tested by ChIP.
Chromatin immunoprecipitation (ChIP) was performed using Active Motif's Epigenetic Services protocol with 30 µg of chromatin from a human B-cell lymphoma cell line and 5 µl of LSD1 antibody. ChIP DNA was used in qPCR with the negative control primer pairs or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Active Motif's Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.
LSD1 antibody (pAb) tested by ChIP-chip.
ChIP was performed using chromatin from a human B cell lymphoma cell line (3 million cells). ChIP DNA was amplified by WGA, labeled and hybridized to a human tiling array. The image on the left shows thousands of LSD1 binding sites across chromosome 4. The image on the right is zoomed in to show a single LSD1 peak in the promoter of the MRPL1 gene.
for LSD1 antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for LSD1 antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
