MeCP2 antibody (pAb)

for MeCP2 antibody (pAb)Background

Methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as development and control of gene expression. It is generally associated with the repressive chromatin state. The complex series of events leading to this repressive state involve the coordinated regulation of DNA methyltransferases and two other groups of proteins called the Methyl-CpG binding proteins (MBD proteins) and the Kaiso family of proteins. The MBD family of proteins include MeCP2, MBD1, MBD2, MBD3 and MBD4. MeCP2 (methyl-CpG binding protein 2) can repress transcription by recruiting the Sin3 complex including histone deacetylases (HDACs). MeCP2 is distributed throughout the nucleus in human cells.

for MeCP2 antibody (pAb)Immunogen

This antibody was raised against recombinant protein corresponding to the amino-terminal 320 amino acids of mouse MeCP2.

for MeCP2 antibody (pAb)Application Notes

This MeCP2 antibody has been validated for use in Western blotting (1:10,000 - 1:50,000 dilution). While a 53 kDa band is predicted, a ~75 kDa band was observed. To avoid detection of cross-reacting proteins, use the antibody at the recommended dilution. MeCP2 is often resolved as a doublet. MeCP2 is subject to a number of different post-translational modifications, including phosphorylation and ubiquitylation. MeCP2 is not easily extractable with non-ionic detergents in the absence of high salt.

for MeCP2 antibody (pAb)Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide.

for MeCP2 antibody (pAb)Positive Control

Active Motif's HeLa acid extract (Catalog No. 36200) can be used as a positive control for MeCP2 antibody.

for MeCP2 antibody (pAb)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for MeCP2 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.