MEP50 antibody (pAb)

for MEP50 antibody (pAb)Background

MEP50 (also designated WDR77) is an important component of the methylosome, a large (20S) protein arginine methyltransferase complex that contains PRMT5. The methylosome functions to modify specific arginines to dimethylarginines in the arginine- and glycine-rich domains of several spliceosomal Sm proteins. This modification targets these proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein (snRNP) core particles. A 50 kDa WD repeat protein MEP50 binds to JBP1 and a subset of Sm proteins and is thought to provide an interface for multiple protein interactions between methylosome proteins. The ability of MEP50 to interact with multiple proteins suggests it is important for targeting the Sm as well as other yet unknown proteins to the methylosome for symmetrical dimethylation. MEP50 specifically interacts with CTD phosphatase FCP1 (CTDP1), a protein that may link the processes of transcriptional elongation and splicing, and with Suz12, a polycomb group protein involved in transcriptional repression.

for MEP50 antibody (pAb)Immunogen

This antibody was raised against full-length human MEP50 with an N-terminal HIS tag.

for MEP50 antibody (pAb)Application Notes

This MEP50 antibody has been validated for use in Western blotting (1:1,000 - 1:2500 dilution). Its specificity was determined by protein competition with recombinant human MEP50. MEP50 migrates at an observed MW of 42 kDa.

for MEP50 antibody (pAb)Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide.

for MEP50 antibody (pAb)Positive Control

Active Motif's HeLa whole-cell extract (Catalog No. 40050) can be used as a positive control for MEP50 antibody.

for MEP50 antibody (pAb)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for MEP50 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.