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APEXL2 antibody (pAb)

Aliases: APEX2, XTH2, ZGRF2
商品コード: 39201 Format: 200 µl ¥67,000 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:54 kDa
Reactivity:Human

Applications

Western Blot Validated

for APEXL2 antibody (pAb)Application Notes


Validated Applications:
WB: 1:500 - 1:1,000 dilution

For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

for APEXL2 antibody (pAb)Immunogen

This APEXL2 antibody was raised against a synthetic peptide corresponding to human APEXL2.

for APEXL2 antibody (pAb)Buffer

PBS containing 0.02% sodium azide. Sodium azide is highly toxic.

for APEXL2 antibody (pAb)References

 
APEXL2 antibody (pAb) tested by Western blot.

APEXL2 pAb tested by Western blot.
APEXL2 detection by Western blot. The analysis of APEXL2 was performed using HeLa whole-cell extract and APEXL2 pAb at a 1:500 dilution.

for APEXL2 antibody (pAb)Background

APEXL2 (APEX2, apurinic endonuclease-like 2 or apyrimidinic endonuclease-like 2) is a multifunctional DNA repair enzyme. The main role of APE is to create a nick in the phosphodiester backbone of the AP site that is created when DNA glycosylase removes the damaged base. Four types of APE (AP endonucleases) are classified according to their site of incision. APEXL2 (APEX2) is a Class II AP endonuclease that cleaves the phosphodiester backbone 5′ to the AP site; it may also be present in the mitochondria.

for APEXL2 antibody (pAb)Positive Control

Active Motif's HeLa whole-cell extract (Catalog No. 40050) can be used as a positive control for APEXL2 antibody.

for APEXL2 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for APEXL2 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

APEXL2 antibody (pAb)
 

Data Thumbnails

APEXL2 antibody (pAb) tested by Western blot.

Western blot of APEXL2 pAb.
(Click image to enlarge and see details.)