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DNMT1 antibody (mAb)

Clone: 60B1220.1
Aliases: AIM, DNMT, MCMT, CXXC9, HSN1E, ADCADN
商品コード: 39204 Format: 100 µg ¥67,000 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG
Purification:Affinity Purified
Host:Mouse
Molecular Weight:190 kDa
Reactivity:Human, Mouse

Applications

ChIP Validated Western Blot Validated Immunoprecipitation Validated Immunohistochemistry Validated

for DNMT1 antibody (mAb) (Clone 60B1220.1)Application Notes


Validated Applications:
WB: 2 - 4 µg/ml dilution

Published Applications:
ChIP
IP
IHC
See references for more information. Individual optimization may be required.

For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

for DNMT1 antibody (mAb) (Clone 60B1220.1)Immunogen

This DNMT1 antibody was raised against a peptide corresponding to amino acid residues 637-651 of human DNMT1.

for DNMT1 antibody (mAb) (Clone 60B1220.1)Buffer

PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.

for DNMT1 antibody (mAb) (Clone 60B1220.1)References

 
DNMT1 antibody (mAb) tested by Western blot.

DNMT1 antibody (mAb) tested by Western blot.
Detection of DNMT1 by Western blot. The analysis was performed using mouse ES whole-cell extract and DNMT1 antibody (mAb) at a 2 µg/ml dilution.

for DNMT1 antibody (mAb) (Clone 60B1220.1)Background

DNMT1DNA methyltransferase 1 (or DNMT 1) proteins are involved in DNA methylation in which a methyl group is added to a cytosine residue on DNA, commonly at the C5 position of a CpG dinucleotide. Three families of DNMTs have been identified: DNMT1, DNMT2, and DNMT3 (comprised of DNMT3A and DNMT3B).

DNMT1 is the most abundant DNMT in somatic cells and shows a preference for methylating hemi-methylated DNA. It is considered to be a maintenance DNA methyltransferase that is important in the maintenance of specific patterns of methylation throughout cellular divisions. Methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as embryonic development, genetic imprinting, X chromosome inactivation and the control of gene expression. DNA methylation is generally associated with transcriptional repression.

for DNMT1 antibody (mAb) (Clone 60B1220.1)Positive Control

Whole-cell extracts from mouse ES cells can be used as a positive control for DNMT1 antibody.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with DNMT1 antibody (mAb).

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use DNMT1 antibody (mAb).

for DNMT1 antibody (mAb) (Clone 60B1220.1)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for DNMT1 antibody (mAb) (Clone 60B1220.1)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

DNMT1 antibody (mAb)
 

Data Thumbnails

DNMT1 antibody (mAb) tested by Western blot.

Western blot of DNMT1 mAb.
(Click image to enlarge and see details.)