DNMT2 antibody (pAb)

for DNMT2 antibody (pAb)Background

DNMT2 – DNA methyltransferase 2 (or DNMT 2) proteins are involved in DNA methylation in which a methyl group is added to a cytosine residue on DNA, commonly at the C5 position of a CpG dinucleotide. Three families of DNMTs have been identified: DNMT1, DNMT2, and DNMT3 (comprised of DNMT3A and DNMT3B).

DNMT2 lacks the large N-terminal regulator domain common to other eukaryotic methyltransferases, but it possess a catalytic domain. It is involved in the methylation of aspartic acid transfer RNA at position 38, giving the enzyme the alternative name of TRDMT1. Although referred to as a DNA methyltransferase, DNMT2 does not methylate DNA, but instead is the first RNA cytosine methyltransferase to be identified.

for DNMT2 antibody (pAb)Immunogen

This antibody was raised against a peptide corresponding to amino acid residues 39-53 and 361-376 of mouse DNMT2.

for DNMT2 antibody (pAb)Application Notes

This DNMT2 antibody has been validated for use in Western blotting (2 - 4 µg/ml dilution). For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

for DNMT2 antibody (pAb)Buffer

PBS containing 0.02% sodium azide. Sodium azide is highly toxic.

for DNMT2 antibody (pAb)Positive Control

Whole-cell extracts from mouse testes can be used as a positive control for DNMT2 antibody.

for DNMT2 antibody (pAb)Storage

Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for DNMT2 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.