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HDAC11 antibody (pAb)

Aliases: HD11
商品コード: 39208 Format: 200 µl ¥67,000 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:36 kDa
Reactivity:Human, Mouse, Rat

Applications

Western Blot Validated

for HDAC11 antibody (pAb)Application Notes


Validated Applications:
WB: 1 - 3 µg/ml dilution

For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

for HDAC11 antibody (pAb)Immunogen

This HDAC11 antibody was raised against a peptide corresponding to amino acid residues 238-251 of human HDAC11.

for HDAC11 antibody (pAb)Buffer

PBS containing 0.2% gelatin and 0.05% sodium azide. Sodium azide is highly toxic.

for HDAC11 antibody (pAb)References

 
HDAC11 antibody (pAb) tested by Western blot.

HDAC11 pAb tested by Western blot.
Detection of HDAC11 by Western blot. The analysis was performed using HDAC11 pAb at a dilution of 2 µg/ml and brain whole-cell extracts from human (lane 1), mouse (lane 2) or rat origin.

for HDAC11 antibody (pAb)Background

HDAC11 (Histone Deacetylase 11) is a member of the class IV mammalian histone deacetylases (HDACs) involved in regulating chromatin structure during transcription. These enzymes catalyze the removal of acetyl groups from lysine residues of histones and other cellular proteins. Lysine N-ε-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in regulation of gene expression in various cellular functions. It consists of the transfer of an acetyl moiety from an acetyl coenzyme A to the ε-amino group of a lysine residue.

In vivo, acetylation is controlled by the antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs). The HDACs are grouped into four classes, on the basis of similarity to yeast counterparts: HDAC class I (HDAC1, HDAC2, HDAC3 and HDAC8), class II (HDAC4, HDAC5, HDAC6, HDAC7, 9 and 10), class III (SIRT1-7) and class IV (HDAC11). In humans, expression of HDAC11 is limited to only a few organs, including brain, heart, muscle and kidney, suggesting an important role of HDAC11 in those tissues.

for HDAC11 antibody (pAb)Positive Control

Active Motif's Mouse Brain nuclear extract (Catalog No. 36053) can be used as a positive control for HDAC11 antibody.

for HDAC11 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for HDAC11 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

HDAC11 antibody (pAb)
 

Data Thumbnails

HDAC11 antibody (pAb) tested by Western blot.

Western blot of HDAC11 pAb.
(Click image to enlarge and see details.)