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INHAT-2/pp32 antibody (pAb)

Aliases: ANP32A, LANP, MAPM, PP32, HPPCn, PHAP1, PHAPI, I1PP2A, C15orf1
商品コード: 39213 Format: 200 µl ¥67,000 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:31 kDa
Reactivity:Human

Applications

Western Blot Validated

for INHAT-2/pp32 antibody (pAb)Application Notes


Validated Applications:
WB: 1:500 - 1:1,000 dilution

For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

for INHAT-2/pp32 antibody (pAb)Immunogen

This INHAT-2/pp32 antibody was raised against a mixture of synthetic peptides corresponding to amino acid residues 87-103 and 231-246 of human INHAT-2/pp32.

for INHAT-2/pp32 antibody (pAb)Buffer

PBS containing 0.02% sodium azide and 0.1 mg/ml BSA. Sodium azide is highly toxic.

for INHAT-2/pp32 antibody (pAb)References

 
INHAT-2/pp32 antibody (pAb) tested by Western blot.

INHAT-2/pp32 pAb tested by Western blot.
Detection of INHAT-2 by Western blot. The analysis was performed using HeLa whole-cell extract and INHAT-2/pp32 pAb at a 1:1,000 dilution.

for INHAT-2/pp32 antibody (pAb)Background

INHAT (pp32) binds to histones and masks them from being substrates for histone acetyltransferases (HATs). Endogenous INHAT subunits, which include the Set/TAF-I beta oncoprotein, and pp32 associate with chromatin in vivo and can block coactivator mediated transcription when transfected in cells. Single phosphorylations within the Histone H3 tail blocks binding of INHAT, as well as simultaneous acetylation of multiple lysine residues.

for INHAT-2/pp32 antibody (pAb)Positive Control

Active Motif's HeLa whole-cell extract (Catalog No. 40050) can be used as a positive control for INHAT-2/pp32 antibody.

for INHAT-2/pp32 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for INHAT-2/pp32 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

INHAT-2/pp32 antibody (pAb)
 

Data Thumbnails

INHAT-2/pp32 antibody (pAb) tested by Western blot.

Western blot of INHAT-2/pp32 pAb.
(Click image to enlarge and see details.)