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Applications Table
| Western Blot | Chromatin IP |
|---|---|
| 1:500 - 1:1,000 | — |
for LIG1 antibody (pAb)Background
LIG1 is an ATP-dependent DNA ligase that participates in base excision and repair processes during DNA replication.
for LIG1 antibody (pAb)Immunogen
This antibody was raised against a peptide corresponding to amino acid residues 51-68 of human LIG1.
for LIG1 antibody (pAb)Application Notes
This LIG1 antibody has been validated for use in Western blotting (1:500 - 1:1,000 dilution). For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.
for LIG1 antibody (pAb)Buffer
PBS containing 0.02% sodium azide and 0.1 mg/ml BSA. Sodium azide is highly toxic.
for LIG1 antibody (pAb)Positive Control
Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for LIG1 antibody.
LIG1 pAb tested by Western blot.
Detection of LIG1 by Western blot. The analysis was performed using HeLa nuclear extract and LIG1 pAb at a 1:1,000 dilution.
for LIG1 antibody (pAb)Storage
Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for LIG1 antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
