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PMS2 antibody (mAb)

Clone: 163C1251
Aliases: PMSL2, HNPCC4, PMS2CL
商品コード: 39221 Format: 100 µg ¥67,000 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG
Purification:Affinity Purified
Host:Mouse
Molecular Weight:85 kDa
Reactivity:Human, Mouse

Applications

Western Blot Validated

for PMS2 antibody (mAb) (Clone 163C1251)Application Notes


Validated Applications:
WB: 1 - 2 µg/ml dilution

For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

for PMS2 antibody (mAb) (Clone 163C1251)Immunogen

This PMS2 antibody was raised against a peptide corresponding to amino acid residues 623-639 of human PMS2.

for PMS2 antibody (mAb) (Clone 163C1251)Buffer

PBS containing 0.02% sodium azide. Sodium azide is highly toxic.

for PMS2 antibody (mAb) (Clone 163C1251)References

 
PMS2 antibody (mAb) tested by Western blot.

PMS2 mAb tested by Western blot.
PMS2 detection by Western blot. The analysis was performed using NIH/3T3 whole-cell extract and PMS2 pAb at a 2 µg/ml dilution.

for PMS2 antibody (mAb) (Clone 163C1251)Background

PMS2Post-meiotic Segregation Increased 2 is the human homolog of the yeast Pms2 protein and is involved in post-replicative DNA mismatch repair (MMR). It forms a heterodimer with MLH-1 and this complex interacts with other protein complexes that recognize and bind to mismatched bases. PMS2 has endonuclease activity and introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation of the existing strand prevents cleavage and dictates that only the unmethylated, newly replicated (and likely to have the incorrect base) strand be repaired. Mutations in this gene are associated with hereditary nonpolyposis colorectal cancer and Turcot syndrome.

for PMS2 antibody (mAb) (Clone 163C1251)Positive Control

Active Motif's NIH/3T3 nuclear extract (Catalog No. 36020) can be used as a positive control for PMS2 antibody.

for PMS2 antibody (mAb) (Clone 163C1251)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for PMS2 antibody (mAb) (Clone 163C1251)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

PMS2 antibody (mAb)
 

Data Thumbnails

PMS2 antibody (mAb) tested by Western blot.

Western blot of PMS2 mAb.
(Click image to enlarge and see details.)