UBE2N antibody (pAb)

for UBE2N antibody (pAb)Background

UBE2N (UBC13, BLU, ubiquitin-conjugating enzyme E2N) heterodimer with UBE2V2 catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through 'Lys-63'. This type of poly-ubiquitination does not lead to protein degradation by the proteasome. UBE2N mediates transcriptional activation of target genes and plays a role in the control of progress through the cell cycle and differentiation. UBE2N also plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage and acts together with the E3 ligase SHPRH in the poly-ubiquitination of PCNA 'Lys-164' upon genotoxic stress, which is required for DNA repair.

for UBE2N antibody (pAb)Immunogen

This antibody was raised against a mixture of synthetic peptides corresponding to amino acid residues 2-19 and 131-148 of human UBE2N.

for UBE2N antibody (pAb)Application Notes

This UBE2N antibody has been validated for use in Western blotting (2 µg/ml), and reported to work in immunoprecipitation. For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

for UBE2N antibody (pAb)Buffer

PBS containing 0.02% sodium azide. Sodium azide is highly toxic.

for UBE2N antibody (pAb)Positive Control

Active Motif's Human Heart nuclear extract (Catalog No. 36041) can be used as a positive control for UBE2N antibody.

for UBE2N antibody (pAb)Storage

Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for UBE2N antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.