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UBE2V1α antibody (pAb)

Aliases: CIR1, UEV1, CROC1, UBE2V, UEV-1, UEV1A, CROC-1
商品コード: 39225 Format: 100 µg ¥67,000 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:20 kDa
Reactivity:Human

Applications

Western Blot Validated

for UBE2V1a antibody (pAb)Application Notes


Validated Applications:
WB: 1 - 3 µg/ml dilution

For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

for UBE2V1a antibody (pAb)Immunogen

This UBE2V1a antibody was raised against a peptide corresponding to amino acid residues 31-49 of human UBE2V1a.

for UBE2V1a antibody (pAb)Buffer

PBS containing 0.2% gelatin and 0.05% sodium azide. Sodium azide is highly toxic.

for UBE2V1a antibody (pAb)References

 
UBE2V1α antibody (pAb) tested by Western blot.

UBE2V1α pAb tested by Western blot.
Detection of UBE2V1α by Western blot. The analysis was performed using HeLa whole-cell extract and UBE2V1α pAb at a 2 µg/ml dilution.

for UBE2V1a antibody (pAb)Background

UBE2N and its variants UBE2V1α and β are members of the E2 ubiquitin-conjugating enzyme family, and the homologs of yeast UBC13. The UBE2V1/UBE2N heterodimer catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through Lys-63. This type of poly-ubiquitination activates IKK (IKKα, IKKβ Clone 10AG2, IKKβ Clone 10A9B6, IKKγ) and does not seem to involve protein degradation by the proteasome. UBE2V1 plays a role in the activation of NFκB (NFκB p50, NFκB p65) mediated by IL-1β, TNF, TRAF6 and TRAF2.

for UBE2V1a antibody (pAb)Positive Control

Active Motif's HeLa whole-cell extract (Catalog No. 40050) can be used as a positive control for UBE2V1α antibody.

for UBE2V1a antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for UBE2V1a antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

UBE2V1α antibody (pAb)
 

Data Thumbnails

UBE2V1α antibody (pAb) tested by Western blot.

Western blot of UBE2V1α pAb.
(Click image to enlarge and see details.)