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JARID1C antibody (pAb)
| Catalog No: 39229 | Format: 200 µl | $340 | Buy Now |
| Catalog No: 39230 | Format: 10 µl | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP |
|---|---|
| 1:500 - 1:2,000 | — |
for JARID1C antibody (pAb)Background
JARID1C is a member of a family of histone demethylase enzymes that contain a Jumonji-N (Jmj-N) and Jumonji-C (Jmj-C) domain in addition to an AT-rich interacting domain (ARID). JARID1C also contains 2 PHD domains and a PLU-1 domain, and a putative DNA binding module. JARID1C demethylates histone H3 that has been di- or trimethylated at lysine 4, and functions as a repressor of transcription. JARID1C also participates in transcriptional repression of neuronal genes by recruiting histone deacetylases and REST at neuron-restrictive silencer elements. Mutations in the JARID1C gene have been associated with X-linked mental retardation.
for JARID1C antibody (pAb)Immunogen
This JARID1C antibody was raised against a peptide corresponding the C-terminus of human JARID1C.
for JARID1C antibody (pAb)Application Notes
This JARID1C antibody has been validated for use in Western blotting (1:500 - 1:2,000 dilution) and chromatin IP (10 µl per ChIP).
for JARID1C antibody (pAb)Buffer
Rabbit serum containing 30% glycerol and 0.035% sodium azide.
for JARID1C antibody (pAb)Positive Control
Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for JARID1C antibody.
Chromatin IP Assays
Active Motif has a variety of Chromatin Immunoprecipitation Kits for every need.
Please visit our Chromatin IP Page to learn about ChIP products designed for use with JARID1C antibody (pAb).
JARID1C pAb tested by Western blot.
Nuclear extract of HeLa cells (20 µg) probed with JARID1C pAb (1:1,000).
JARID1C pAb tested by ChIP.
Chromatin immunoprecipitation (ChIP) was performed using Active Motif's Epigenetic Services protocol with 30 µg of chromatin from human MIA PaCa-2 pancreatic carcinoma cells and 10 µl JARID1C antibody. ChIP DNA was used in qPCR with the control primer pairs or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Active Motif's Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.
for JARID1C antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for JARID1C antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
