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Dimethyl-Arginine, asymmetric antibody (pAb)
| Catalog No: 39231 | Format: 200 µl | $340 | Buy Now |
| Catalog No: 39232 | Format: 10 µl | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP | Immunofluorescence |
|---|---|---|
| 1:500 - 1:2,000 | — | 1:250 - 1:500 |
for Dimethyl-Arginine, asymmetric antibody (pAb)Background
Dimethyl-Arginine, asymmetric (or Asymmetric dimethylarginine, ADMA) interferes with the production of nitric oxide (NO). Arginine methylation is an important post-translational modification catalyzed by the PRMT/CARM family of methyltransferases.
for Dimethyl-Arginine, asymmetric antibody (pAb)Immunogen
This Dimethyl-Arginine, asymmetric antibody was raised against peptide containing dimethyl-arginine (asymmetric).
for Dimethyl-Arginine, asymmetric antibody (pAb)Application Notes
This Dimethyl-Arginine, asymmetric antibody has been validated for use in Western blotting (1:500 - 1:2,000 dilution) and immunofluorescence (1:250 - 1:500 dilution).
for Dimethyl-Arginine, asymmetric antibody (pAb)Buffer
Rabbit serum containing 30% glycerol and 0.035% sodium azide.
for Dimethyl-Arginine, asymmetric antibody (pAb)Positive Control
Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for Dimethyl-Arginine, asymmetric antibody.
Compatible Secondary Antibodies
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Dimethyl-Arginine, asymmetric antibody (pAb).
Dimethyl-Arginine, asymmetric pAb tested by Western blot.
Detection of Dimethyl-Arginine by Western blot. The analysis was performed using 20 µg HeLa nuclear extract and Dimethyl-Arginine, asymmetric pAb at a 1:1,000 dilution.
Dimethyl-Arginine, asymmetric pAb tested by immunofluorescence.
Staining of HeLa cells with Dimethyl-Arginine, asymmetric pAb (1:250 dilution, top panel) and DAPI (middle panel), and a merge of both images (bottom panel).
for Dimethyl-Arginine, asymmetric antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Dimethyl-Arginine, asymmetric antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
