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Histone H3 dimethyl Lys36 antibody (pAb)
| Catalog No: 39255 | Format: 200 µl | $340 | Buy Now |
| Catalog No: 39256 | Format: 10 µl | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP | ChIP-Seq / ChIP-chip |
IF |
|---|---|---|---|
| 1:2,500 - 1:5,000 | 3 - 5 µl per ChIP | 10 µl per ChIP-Seq 10 µl per ChIP-chip |
1:250 - 1:1,000 |
for Histone H3 dimethyl Lys36 antibody (pAb)Background
Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.
The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Histone H3 is methylated at lysine 36 by the Set2 (yeast) and NSD1 (mammals) methyltransferases. Dimethylation of lysine 36 of histone H3 is involved with transcriptional elongation by RNA pol II holoenzyme and is a marker of transcribed genes.
for Histone H3 dimethyl Lys36 antibody (pAb)Immunogen
This Histone H3 dimethyl Lys36 antibody was raised against a peptide including dimethyl-lysine 36 of histone H3.
for Histone H3 dimethyl Lys36 antibody (pAb)Application Notes
This Histone H3 dimethyl Lys36 antibody (H3K36me2) has been validated for use in chromatin IP (3 - 5 µl per ChIP), ChIP-Seq & ChIP-chip (10 µl each), Western blotting (1:2,500 - 1:5,000 dilution) and immunofluorescence (1:250 - 1:1,000 dilution).
Detection of histone H3 with this antibody disappears completely in Western blots of a yeast strain deleted for the Set2 gene, which encodes for the Lys36 methyltransferase.
for Histone H3 dimethyl Lys36 antibody (pAb)Buffer
Rabbit serum containing 30% glycerol and 0.035% sodium azide.
for Histone H3 dimethyl Lys36 antibody (pAb)Positive Control
Active Motif's HeLa acid extract (Catalog No. 36200) can be used as a positive control for Histone H3 dimethyl Lys36 antibody.
Chromatin IP Assays
Active Motif has a variety of Chromatin Immunoprecipitation Kits for every need.
Please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H3 dimethyl Lys36 antibody (pAb).
Compatible Secondary Antibodies
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Histone H3 dimethyl Lys36 antibody (pAb).
Histone H3 dimethyl Lys36 antibody tested by Western blot.
HeLa acid extract (10 µg per lane) probed with Histone H3 dimethyl Lys36 antibody at a dilution of 1:2,500.
Histone H3 dimethyl Lys36 antibody tested by immunofluorescence.
Staining of HeLa cells with Histone H3 dimethyl Lys36 antibody (1:500 dilution, top panel) and DAPI (middle panel), and a merge of both images (bottom panel).
Histone H3 dimethyl Lys36 antibody tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H3 dimethyl Lys36 antibody for histone H3 dimethyl Lys36. Methylated peptides corresponding to the immunogen were spotted onto PVDF and probed with the antibody at 1:5,000. The amount of peptide (picomoles) spotted is indicated next to each row.
Lane 1: trimethyl-Lys36 peptide.
Lane 2: dimethyl-Lys36 peptide.
Lane 3: unmodified Lys36 peptide.
Histone H3 dimethyl Lys36 antibody tested by ChIP-chip.
ChIP was performed using chromatin from a human lymphoma cell line (3 million cells). The ChIP DNA was amplified by WGA, labeled and hybridized to a human tiling array. The image shows H3K36me2 enrichment across the FMNL2 gene.
for Histone H3 dimethyl Lys36 antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Histone H3 dimethyl Lys36 antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
