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Aurora B antibody (pAb)
| RRID: AB_2793209 |
| Aliases: Aurora Kinase B, AurB, ARK2 |
| Catalog No: 39261 | Format: 200 µl | ¥86,000 | Buy |
| Catalog No: 39262 | Format: 10 µl | ¥24,000 | Buy |
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Applications
Application Notes
Applications Validated by Active Motif:
ICC/IF: 1:200 - 1:1,000 dilution
WB: 1:500 - 1:5,000 dilution
Immunogen
This Aurora B antibody was raised against a peptide including the C-terminus of human Aurora B.
Buffer
Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Aurora B pAb tested by immunofluorescence.
Top left: HeLa cells stained with Aurora B polyclonal antibody at a 1:200 dilution. Top right: Same cells stained with alpha Tubulin mAb. Bottom left: Same cells stained with DAPI. Bottom right: Merge of all 3 images.
Aurora B pAb tested by Western blot
Nuclear extract of U2OS cells (20 µg per lane) probed with Aurora B polyclonal antibody (1:500 dilution).
Background
Aurora B (Aurora Kinase B, AurB, ARK2) is a Ser/Thr kinase that is part of the chromosomal passenger complex that associates with chromosomes and is an important regulator of mitosis and meiosis. Aurora B becomes concentrated at the centromere during the onset of mitosis, but relocates to the spindle midzone during anaphase. Aurora B phosphorylates a number of substrates, including INCENP, CENP-A, and Histone H3. Misregulation of Aurora B activity can lead to chromosome instability and is implicated in a number of cancers.
Positive Control
Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for Aurora B antibody.
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- WB = Western Blotting
