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Antibodies

Jhd2 antibody (pAb)

Catalog No: 39263 Format: 200 µl $340 Buy Now
Catalog No: 39264 Format: 10 µl $70 Buy Now
Antibody Type:Polyclonal
Host:Rabbit
Isotype:IgG
Purification:Affinity Purified
Applications:WB
Reactivity:Budding Yeast
Molecular Weight:85 kDa

Applications Table

Western Blot Chromatin IP
1:250 - 1:1,000

for Jhd2 antibody (pAb)Background

Jhd2 is a JmjC domain family histone demethylase specific for H3K4 (lysine at position 4 of the histone H3 protein). It removes methyl groups specifically added by Set1p methyltransferase.

for Jhd2 antibody (pAb)Immunogen

This antibody was raised against a peptide corresponding to the C-terminus of Jhd2 from S. cerevisiae.

for Jhd2 antibody (pAb)Application Notes

This Jhd2 antibody has been validated for use in Western blotting (1:250 - 1:1,000 dilution).

for Jhd2 antibody (pAb)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol , 0.7mg/ml BSA and 0.035% sodium azide.

for Jhd2 antibody (pAb)Positive Control

Whole-cell extracts from yeast can be used as positive control for Jhd2 antibody.

 
Jhd2 pAb tested by Western blot.

Jhd2 pAb tested by Western blot.
S. cerevesiae extracts probed with Jhd2 pAb (1:1,000 dilution).
   Lane 1: wild type strain.
   Lane 2: Jhd2 knockout strain.

for Jhd2 antibody (pAb)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Jhd2 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FACS = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • WB = Western Blotting

Technical Data Sheet

Jhd2 antibody (pAb)
 

Data Thumbnails

Jhd2 pAb tested by Western blot.

Western blot of Jhd2 pAb.
(Click image to enlarge and see details.)