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Histone H3K56ac antibody (pAb)
| RRID: AB_2661786 |
| 商品コード: 39281 | Format: 100 µl | ¥92,000 | Buy |
| 商品コード: 39082 | Format: 50 µl | ¥55,000 | Buy |
| 商品コード: 39282 | Format: 10 µl | ¥25,000 | Buy |
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Applications
Published Applications
The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:
ChIP, ChIP-Seq
WB
View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.
Immunogen
This Histone H3 acetyl Lys56 antibody was raised against peptide containing acetyl-Lys56 of yeast histone H3.
Buffer
Purified rabbit IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Histone H3K56ac antibody (pAb) tested by ChIP-chip.
ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with chromatin from 3 million HeLa cells and 5 µl of antibody. ChIP DNA was amplified by WGA, labeled and hybridized to a human tiling array. The image on the left shows H3K56Ac binding across a 650,000 bp region on chromosome 17. The image on the right shows H3K56Ac binding at the KLF5 gene.
Histone H3K56ac antibody (pAb) tested by ChIP analysis.
Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) using 2 µl of Histone H3 acetyl Lys56 pAb or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the indicated gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.
Histone H3K56ac antibody tested by Western blot.
HeLa acid extract (20 µg/lane) was probed with Histone H3 acetyl Lys56 polyclonal antibody (1:5,000 dilution).
Lane 1: No treatment.
Lane 2: Cells treated with sodium butyrate.
Histone H3K56ac antibody (pAb) tested by CUT&Tag
CUT&Tag was performed using 100,000 K562 cells and sequenced using 38 base-pair, paired-end reads on the Illumina NovaSeq. Data was collected from 7 million reads, and H3K56ac data is shown for Chromosome 1.
Histone H3K56ac antibody (pAb) tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H3 acetyl Lys56 pAb for acetyl Lys56 histone H3. Acetylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at 1:20,000. The amount of peptide (picomoles) spotted is indicated next to each row.
Lane 1: H3 acetyl-Lys4 peptide. Lane 2: H3 acetyl-Lys9 peptide. Lane 3: H3 acetyl-Lys14 peptide. Lane 4: H3 acetyl-Lys18 peptide.. Lane 5: unmodified H3 peptide aa 13-22. Lane 6: H3 acetyl-Lys23 peptide. Lane 7: H3 acetyl-Lys27 peptide. Lane 8: H3 acetyl-Lys36 peptide. Lane 9: H3 acetyl-Lys37 peptide. Lane 10: yeast H3 acetyl-Lys56 peptide. Lane 11: unmodified yeast H3 peptide aa 50-59. Lane 12: human H3 acetyl-Lys56 peptide.
Chromatin IP Assays
This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H3K56ac antibody (pAb).
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
- WB = Western Blotting
