AML-3/Runx2 antibody (pAb)

RRID: AB_2793223
Aliases: CCD, CCD1, CLCD, OSF2, CBFA1, OSF-2, PEA2aA, PEBP2aA, CBF-alpha-1
Catalog No: 39302 Format: 100 µl Discontinued

Antibody Type:
Polyclonal
Isotype:
Serum
Purification:
None
Host:
Rabbit
Molecular Weight:
56 kDa
Reactivity:
Human

Applications

Western Blot Validated Electrophoretic Mobility Shift Assay Validated Immunocytochemistry Validated

Application Notes

This product has been discontinued

Applications Validated by Active Motif:
EMSA: 2 - 6 µl per assay

The addition of Tris-glycine electrophoresis buffer for supershift assays is recommended.

Published Applications

The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:

EMSA
ICC
WB

Immunogen

This AML-3/Runx2 antibody was raised against a synthetic peptide corresponding to amino acid residues 333-348 of human AML-3/Runx2.

Buffer

PBS containing 0.2% gelatin, 0.01% thimersol and 0.1% sodium azide. Thimersol and sodium azide are highly toxic.

 

AML-3/Runx2 pAb tested by supershift.
Detection of AML-3/Runx2 by EMSA analysis. AML-3/Runx2 pAb was used in a supershift assay (EMSA) with Saos-2 nuclear extract (lane 4).

Background

AML-3 (acute myeloid leukemia 3 protein) also known as Runx2 (runt-related transcription factor 2) is a multifunctional transcription factor that can either activate or repress transcription. The alpha subunit can bind DNA as a monomer, or DNA binding can be increased by heterodimerization. AML-3 is necessary for osteoblastic differentiation and skeletal morphogenesis.

Positive Control

Active Motif's Saos-2 nuclear extract (Catalog No. 36025) can be used as a positive control for AML-3/Runx2 antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting