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AML-3/Runx2 antibody (pAb)

Aliases: CCD, CCD1, CLCD, OSF2, CBFA1, OSF-2, PEA2aA, PEBP2aA, CBF-alpha-1
商品コード: 39302 Format: 100 µl ¥67,000 Buy Now

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Antibody Type:Polyclonal
Isotype:Serum
Purification:None
Host:Rabbit
Molecular Weight:56 kDa
Reactivity:Human

Applications

Electrophoretic Mobility Shift Assay Validated

for AML-3/Runx2 antibody (pAb)Application Notes


Validated Applications:
EMSA: 2 - 6 µl per assay

Published Applications: see references

The addition of Tris-glycine electrophoresis buffer for supershift assays is recommended.

for AML-3/Runx2 antibody (pAb)Immunogen

This AML-3/Runx2 antibody was raised against a synthetic peptide corresponding to amino acid residues 333-348 of human AML-3/Runx2.

for AML-3/Runx2 antibody (pAb)Buffer

PBS containing 0.2% gelatin, 0.01% thimersol and 0.1% sodium azide. Thimersol and sodium azide are highly toxic.

for AML-3/Runx2 antibody (pAb)References

 
AML-3/Runx2 antibody (pAb) tested by supershift.

AML-3/Runx2 pAb tested by supershift.
Detection of AML-3/Runx2 by EMSA analysis. AML-3/Runx2 pAb was used in a supershift assay (EMSA) with Saos-2 nuclear extract (lane 4).

for AML-3/Runx2 antibody (pAb)Background

AML-3 (acute myeloid leukemia 3 protein) also known as Runx2 (runt-related transcription factor 2) is a multifunctional transcription factor that can either activate or repress transcription. The alpha subunit can bind DNA as a monomer, or DNA binding can be increased by heterodimerization. AML-3 is necessary for osteoblastic differentiation and skeletal morphogenesis.

for AML-3/Runx2 antibody (pAb)Positive Control

Active Motif's Saos-2 nuclear extract (Catalog No. 36025) can be used as a positive control for AML-3/Runx2 antibody.

for AML-3/Runx2 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for AML-3/Runx2 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

AML-3/Runx2 antibody (pAb)
 

Data Thumbnails

AML-3/Runx2 antibody (pAb) tested by supershift.

Supershift of AML-3/Runx2 pAb.
(Click image to enlarge and see details.)