C/EBPα antibody (pAb)

for C/EBPa antibody (pAb)Background

C/EBPα (or C/EBP alpha) is a member of the CCAAT-enhancer-binding protein family of transcription factors. C/EBPs can bind DNA and recruit coactivators to promote gene expression. C/EBPs contains a basic-leucine zipper (bZIP) domain and can bind as homodimers to promoters. C/EBPα can also heterodimerize with C/EBPβ and C/EBPγ.

for C/EBPa antibody (pAb)Immunogen

This C/EBPa antibody was raised against a synthetic peptide corresponding to amino acid residues 1-14 of human C/EBP alpha.

for C/EBPa antibody (pAb)Application Notes

This C/EBPa antibody has been validated for use in chromatin IP (5 µl per ChIP), Western blotting (1:1,000 dilution) and electrophoretic mobility shift assays (EMSA), and it has been reported to work in immunofluorescence. We recommend adding 2 - 4 µl of antibody to the reaction mixture when performing supershift experiments, and also recommend the use of Tris-glycine electrophoresis buffer. For optimal results in Western blot experiments, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

for C/EBPa antibody (pAb)Buffer

PBS containing 0.2% gelatin, 0.01% thimersol and 0.1% sodium azide. Thimersol and sodium azide are highly toxic.

for C/EBPa antibody (pAb)Positive Control

Active Motif's Rat Liver nuclear extract (Catalog No. 36024) can be used as a positive control for C/EBPα antibody.

for C/EBPa antibody (pAb)Storage

Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for C/EBPa antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.