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c-Jun antibody (pAb)

Aliases: AP1, AP-1, JUN
商品コード: 39309 Format: 100 µg ¥67,000 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:41 kDa
Reactivity:Human, Mouse

Applications

ChIP Validated Electrophoretic Mobility Shift Assay Validated

for c-Jun antibody (pAb)Application Notes


Validated Applications:
EMSA: 6 µl per assay

The addition of Tris-boric electrophoresis buffer for supershift assays is recommended. Individual optimization may be required.

Published Applications:
ChIP

for c-Jun antibody (pAb)Immunogen

This c-Jun antibody was raised against a synthetic peptide corresponding to amino acid residues 20-37 of human c-Jun.

for c-Jun antibody (pAb)Buffer

PBS containing 0.2% gelatin, 0.01% thimersol and 0.1% sodium azide. Thimersol and sodium azide are highly toxic.

for c-Jun antibody (pAb)References

 
c-Jun antibody (pAb) tested by supershift.

c-Jun antibody tested by supershift.
Detection of c-Jun by EMSA analysis. c-Jun antibody was used in a supershift assay (EMSA) with 3T6 Swiss albino nuclear extract (lane 4). Lane 5 demonstrates a supershift competition in the presence of neutralizing peptide.

for c-Jun antibody (pAb)Background

c-Jun is a component of the AP-1 transcription factor complex in combination with c-Fos. It can be activated by phosphorylation from the JNK pathway.

for c-Jun antibody (pAb)Positive Control

Active Motif's K-562 nuclear extract (TPA stimulated) (Catalog No. 36070) can be used as a positive control for c-Jun antibody.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with c-Jun antibody (pAb).

for c-Jun antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for c-Jun antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

c-Jun antibody (pAb)
 

Data Thumbnails

c-Jun antibody (pAb) tested by supershift.

Supershift of c-Jun pAb.
(Click image to enlarge and see details.)