Your Cart
Browse
Filter
Histone H3 dimethyl Lys14 antibody (pAb)
| Catalog No: 39349 | Format: 200 µl | $340 | Buy Now |
| Catalog No: 39350 | Format: 10 µl | $70 | Buy Now |
|
|
Applications Table
| Western Blot | Chromatin IP |
|---|---|
| 1:500 - 1:2,000 | — |
for Histone H3 dimethyl Lys14 antibody (pAb)Background
Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.
The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue.
for Histone H3 dimethyl Lys14 antibody (pAb)Immunogen
Peptide including dimethyl-lysine 14 of human histone H3.
for Histone H3 dimethyl Lys14 antibody (pAb)Application Notes
This Histone H3 dimethyl Lys14 antibody (H3K14me2) has been validated for use in Western blotting (1:500 - 1:2,000 dilution).
for Histone H3 dimethyl Lys14 antibody (pAb)Buffer
Rabbit serum containing 30% glycerol, 0.035% sodium azide.
for Histone H3 dimethyl Lys14 antibody (pAb)Positive Control
Active Motif's HeLa acid extract (Catalog No. 36200) can be used as a positive control for Histone H3 dimethyl Lys14 antibody.
Histone H3 dimethyl Lys14 pAb tested by Western blot.
Detection of dimethylated Histone H4 by Western blot. The analysis was performed using 20 µg HeLa acid extract (10 µg, lane 1) and Histone H3 dimethyl Lys14 pAb at a 1:500 dilution.
Histone H3 dimethyl Lys14 pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H3 dimethyl Lys14 pAb (Cat. No. 39349) for histone H3 dimethylated at Lys14. Methylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at a dilution of 1:5,000. The amount of peptide (picomoles) spotted is indicated in the left lane next to each row.
Top image:
Lane 1: Unmodified lysine 4 peptide.
Lane 2: Monomethyl lysine 4 peptide.
Lane 3: Dimethyl lysine 4 peptide.
Lane 4: Trimethyl lysine 4 peptide.
Lane 5: Monomethyl lysine 4 peptide #2.
Lane 6: Unmodified peptide corresponding to amino acids 6-22 of human histone H3.
Lane 7: Monomethyl lysine 9 peptide.
Lane 8: Dimethyl lysine 9 peptide.
Lane 9: Trimethyl lysine 9 peptide.
Bottom image:
Lane 1: Unmodified lysine 27 peptide.
Lane 2: Monomethyl lysine 27 peptide.
Lane 3: Dimethyl lysine 27 peptide.
Lane 4: Trimethyl lysine 27 peptide.
Lane 5: Monomethyl lysine 14 peptide #2.
Lane 6: Dimethyl lysine 14 peptide.
No detection of peptides (mono-, di-, or trimethylated) corresponding to lysine 56 of histone H3 was observed (data not shown).
for Histone H3 dimethyl Lys14 antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Histone H3 dimethyl Lys14 antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
