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SIRT1 antibody (mAb)
| Clone: 2G1/F7 |
| Catalog No: 39353 | Format: 200 µg | $340 | Buy Now |
| Catalog No: 39354 | Format: 10 µg | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP | IF | IP |
|---|---|---|---|
| 0.5 - 2 µg/ml | — | Published. See Application Notes below. |
Published. See Application Notes below. |
for SIRT1 antibody (mAb) (Clone 2G1/F7)Background
SIRT1 (Sirtuin 1, SIR2L1) is a member of the Sirtuin family of proteins related to the yeast Sir2 protein. Like Sir2 and other Sirtuins, SIRT1 is an NAD-dependent histone and protein deacetylase. SIRT1 catalyzes the removal of acetyl groups from a variety of protein substrates (NBS1, p53, Ku70, etc.) as well as from Histone H4 Lys16. SIRT1 is involved in the regulation of a number of cellular processes, including transcription, metabolism, DNA repair and aging, and increased SIRT1 is associated with extended lifespan of C. elegans.
for SIRT1 antibody (mAb) (Clone 2G1/F7)Immunogen
This SIRT1 antibody was raised against a synthetic peptide corresponding to amino acids 581-630 of human SIRT1.
for SIRT1 antibody (mAb) (Clone 2G1/F7)Application Notes
This SIRT1 antibody has been validated for use in Western blotting (0.5 - 2 µg/ml), and reported to work in immunofluorescence and immunoprecipitation (see references). Addition of 0.05% Tween 20 in blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot.
for SIRT1 antibody (mAb) (Clone 2G1/F7)Buffer
10 mM sodium phosphate pH 7.5, 150 mM NaCl, 30% glycerol, 0.035% sodium azide.
for SIRT1 antibody (mAb) (Clone 2G1/F7)Positive Control
Nuclear extracts from CMSL0 cells can be used as positive control for SIRT1 antibody (Clone 2G1/F7).
Bridging Antibody to Improve ChIP, MeDIP and IP
Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use SIRT1 antibody (mAb).
Compatible Secondary Antibodies
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with SIRT1 antibody (mAb).
for SIRT1 antibody (mAb) (Clone 2G1/F7)References
This antibody has been used in the following reference:
Vaquero A et al (2004) Mol. Cell 16:93-105.
SIRT1 mAb (Clone 2G1/F7) tested by Western blot.
Nuclear extract (20 µg) of CMSL0 cells stained with SIRT1 mAb (Clone 2G1/F7) at a dilution of 1 µg/ml.
for SIRT1 antibody (mAb) (Clone 2G1/F7)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for SIRT1 antibody (mAb) (Clone 2G1/F7)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
