Ikaros antibody (mAb)

for Ikaros antibody (mAb) (Clone 2A9)Background

Ikaros is a transcription factor that plays a critical role in the control of lymphohematopoiesis and immune regulation. All Ikaros isoforms share a common C-terminal domain that contains a transcription activation motif and two zinc finger motifs required for hetero- and homo-dimerization among the Ikaros isoforms, and for interactions with other proteins. Ikaros is abundantly expressed in pituitary mammosomatotrophs, where it deacetylates Histone H3 sites on the proximal growth hormone (GH) promoter to silence gene expression.

for Ikaros antibody (mAb) (Clone 2A9)Immunogen

This Ikaros antibody was raised against a recombinant protein corresponding to the N-terminal half of mouse Ikaros.

for Ikaros antibody (mAb) (Clone 2A9)Application Notes

This Ikaros antibody has been validated for use in Western blotting (0.5 - 2 µg/ml dilution). This antibody recognizes the largest isoform of Ikaros.

for Ikaros antibody (mAb) (Clone 2A9)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide.

for Ikaros antibody (mAb) (Clone 2A9)Positive Control

Whole-cell extracts from mouse spleen can be used as a positive control for Ikaros antibody (Clone 2A9).

for Ikaros antibody (mAb) (Clone 2A9)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Ikaros antibody (mAb) (Clone 2A9)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.