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Histone H3 monomethyl Lys122 antibody (pAb)
| Catalog No: 39367 | Format: 200 µl | $340 | Buy Now |
| Catalog No: 39368 | Format: 10 µl | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP |
|---|---|
| 1:250 - 1:1,000 | — |
for Histone H3 monomethyl Lys122 antibody (pAb)Background
Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.
The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue.
for Histone H3 monomethyl Lys122 antibody (pAb)Immunogen
Peptide including monomethyl-lysine 122 of human histone H3.
for Histone H3 monomethyl Lys122 antibody (pAb)Application Notes
This Histone H3 monomethyl Lys122 antibody (H3K122me) has been validated for use in Western blotting (1:250 - 1:1,000 dilution).
for Histone H3 monomethyl Lys122 antibody (pAb)Buffer
Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide.
for Histone H3 monomethyl Lys122 antibody (pAb)Positive Control
Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for Histone H3 monomethyl Lys122 antibody.
Histone H3 monomethyl Lys122 pAb tested by Western blot.
Nuclear extract of HeLa cells (20 µg) was probed with Histone H3 monomethyl Lys122 pAb (1:500 dilution).
Histone H3 monomethyl Lys122 pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of 39367 for monomethyl Lys122 histone H3. Methylated peptides corresponding to the immunogen were spotted onto PVDF and probed with 39367 at 1:5,000. The amount of peptide (picomoles) spotted is indicated next to each row.
Lane 1: unmodified Lys122 H3 peptide.
Lane 2: monomethyl-Lys122 H3 peptide.
Lane 3: dimethyl-Lys122 H3 peptide.
Lane 4: trimethyl-Lys122 H3 peptide.
for Histone H3 monomethyl Lys122 antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Histone H3 monomethyl Lys122 antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
