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Histone H4K31me1 antibody (pAb)

商品コード: 39385 Format: 200 µl ¥67,000 Buy Now
商品コード: 39386 Format: 10 µl ¥19,000 Buy Now

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Antibody Type:Polyclonal
Isotype:Serum
Purification:None
Host:Rabbit
Molecular Weight:8 kDa
Reactivity:Human, Other (Wide Range)

Applications

Western Blot Validated Immunofluorescence Validated Dot Blot Validated

for Histone H4 monomethyl Lys31 antibody (pAb)Application Notes


Validated Applications:
WB: 1:500 - 1:2,000 dilution

Published Applications:
IF

for Histone H4 monomethyl Lys31 antibody (pAb)Immunogen

This Histone H4 monomethyl Lys31 antibody was raised against a peptide containing monomethyl Lys31 of human histone H4.

for Histone H4 monomethyl Lys31 antibody (pAb)Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for Histone H4 monomethyl Lys31 antibody (pAb)References

 
Histone H4K31me1 antibody (pAb) tested by Western blot.

Histone H4 monomethyl Lys31 pAb tested by Western blot.
HeLa cell nuclear extract (20 µg per lane) was probed with Histone H4 monomethyl Lys31 pAb at a dilution of 1:1,000.

Histone H4K31me1 antibody (pAb) tested by dot blot analysis.

Histone H4 monomethyl Lys31 pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H4 monomethyl Lys31 pAb for monomethyl Lys31 histone H4. Methylated peptides corresponding to the immunogen were spotted onto PVDF and probed with the antibody at 1:5,000. The amount of peptide (picomoles) spotted is indicated next to each row.
    Lane 1: Unmodified peptide corresponding to amino acids 28-36 of human histone H4
    Lane 2: Monomethyl Lys31
    Lane 3: Dimethyl Lys31; Lane 4: Trimethyl Lys31
    Lane 5: Unmodified peptide corresponding to amino acids 41-49 of human histone H4
    Lane 6: Monomethyl Lys44
    Lane 7: Dimethyl-Lys44
    Lane 8: Trimethyl Lys44.
No detection of peptides (mono-, di-, or trimethylated) corresponding to lysine 4, 9, 18, 23 or 27 of histone H3 was observed with this antibody (data not shown).

for Histone H4 monomethyl Lys31 antibody (pAb)Background

Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.

The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue.

for Histone H4 monomethyl Lys31 antibody (pAb)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for Histone H4 monomethyl Lys31 antibody.

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Histone H4K31me1 antibody (pAb).

for Histone H4 monomethyl Lys31 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Histone H4 monomethyl Lys31 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Histone H4K31me1 antibody (pAb)
 

Data Thumbnails

Histone H4K31me1 antibody (pAb) tested by Western blot.

Western blot of Histone H4K31me1 pAb.
(Click image to enlarge and see details.)

Histone H4K31me1 antibody (pAb) tested by dot blot analysis.

Dot blot of Histone H4K31me1 pAb.
(Click image to enlarge and see details.)