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Histone H3 monomethyl Lys23 antibody (pAb)
| Catalog No: 39387 | Format: 200 µl | $340 | Buy Now |
| Catalog No: 39388 | Format: 10 µl | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP |
|---|---|
| 1:1,000 - 1:5,000 | — |
for Histone H3 monomethyl Lys23 antibody (pAb)Background
Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.
The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue.
for Histone H3 monomethyl Lys23 antibody (pAb)Immunogen
Peptide including monomethyl Lys23 of human histone H3.
for Histone H3 monomethyl Lys23 antibody (pAb)Application Notes
This Histone H3 monomethyl Lys23 antibody (H3K23me) has been validated for use in Western blotting (1:1,000 - 1:5,000 dilution). It was immunodepleted over a peptide column to remove reactivity to di- and tri-methyl H3 Lys23.
for Histone H3 monomethyl Lys23 antibody (pAb)Buffer
Rabbit serum containing 30% glycerol and 0.035% sodium azide.
for Histone H3 monomethyl Lys23 antibody (pAb)Positive Control
Active Motif's HeLa acid extract (Catalog No. 36200) can be used as a positive control for Histone H3 monomethyl Lys23 antibody.
Histone H3 monomethyl Lys23 pAb tested by Western blot.
HeLa cell nuclear extract (20 µg per lane) was probed with Histone H3 monomethyl Lys23 pAb at a dilution of 1:1,000.
Histone H3 monomethyl Lys23 pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H3 monomethyl Lys23 pAb for monomethyl Lys23 histone H3. Methylated peptides corresponding to the immunogen and related sequences derived from histone H3 were spotted onto PVDF and probed with the antibody at 1:5,000. The amount of peptide (picomoles) spotted is indicated next to each row.
Top row
Lane 1: Unmodified peptide corresponding to amino acids 1-10 of human histone H3.
Lane 2: Monomethyl lysine 4 peptide.
Lane 3: Dimethyl lysine 4 peptide.
Lane 4: Trimethyl lysine 4 peptide.
Lane 5: Unmodified peptide corresponding to amino acids 6-22 of human histone H3.
Lane 6: Monomethyl lysine 9 peptide.
Lane 7: Dimethyl lysine 9 peptide.
Lane 8: Trimethyl lysine 9 peptide.
Bottom row
Lane 1: Dimethyl lysine 14 peptide.
Lane 2: Monomethyl lysine 18 peptide.
Lane 3: Dimethyl lysine 18 peptide.
Lane 4: Trimethyl lysine 18 peptide.
Lane 5: Unmodified peptide corresponding to amino acids 18-27 of human histone H3.
Lane 6: Monomethyl lysine 23 peptide.
Lane 7: Dimethyl lysine 23 peptide.
Lane 8: Trimethyl lysine 23 peptide.
Lane 9: Unmodified peptide corresponding to amino acids 22-32 of human histone H3.
Lane 10: Monomethyl lysine 27 peptide.
Lane 11: Dimethyl lysine 27 peptide.
Lane 12: Trimethyl lysine 27 peptide.
for Histone H3 monomethyl Lys23 antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Histone H3 monomethyl Lys23 antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
